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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Biochem J. 1965 Jul;96(1):266-9. doi: 10.1042/bj0960266.

Ribosomal RNA has been prepared by extracting tissues with a phenol-cresol mixture, and ribosomal RNA can be selectively precipitated with m-cresol. No rapidly labelled RNA was associated with this material. 2. However, if RNA and DNA are extracted with 4-aminosalicylate and phenol-cresol mixture and the nucleic acids precipitated, DNA, glycogen and s-RNA (transfer RNA) can be extracted with 3m-sodium acetate and in this case rapidly labelled RNA remains associated with the ribosomal RNA. 3. The ribosomal RNA is stable in the presence of concentrated salt solution and, although the secondary structure is lost by heating at 70 degrees in 10mm-sodium acetate, it can be re-formed in the presence of 200mm-sodium acetate. 4. The 28s and 18s components have been separated and their base compositions determined.

通过用苯酚 - 甲酚混合物提取组织来制备核糖体RNA，并且核糖体RNA可以用间甲酚选择性沉淀。没有快速标记的RNA与这种物质相关联。2. 然而，如果用对氨基水杨酸盐和苯酚 - 甲酚混合物提取RNA和DNA并沉淀核酸，DNA、糖原和s - RNA（转移RNA）可以用3m醋酸钠提取，在这种情况下，快速标记的RNA仍然与核糖体RNA相关联。3. 核糖体RNA在浓盐溶液存在下是稳定的，尽管在10mm醋酸钠中于70度加热会使其二级结构丧失，但在200mm醋酸钠存在下它可以重新形成。4. 28s和18s组分已被分离并测定了它们的碱基组成。

Biochem J. 1965 Jul;96(1):266-9. doi: 10.1042/bj0960266.

Ribosomal RNA has been prepared by extracting tissues with a phenol-cresol mixture, and ribosomal RNA can be selectively precipitated with m-cresol. No rapidly labelled RNA was associated with this material. 2. However, if RNA and DNA are extracted with 4-aminosalicylate and phenol-cresol mixture and the nucleic acids precipitated, DNA, glycogen and s-RNA (transfer RNA) can be extracted with 3m-sodium acetate and in this case rapidly labelled RNA remains associated with the ribosomal RNA. 3. The ribosomal RNA is stable in the presence of concentrated salt solution and, although the secondary structure is lost by heating at 70 degrees in 10mm-sodium acetate, it can be re-formed in the presence of 200mm-sodium acetate. 4. The 28s and 18s components have been separated and their base compositions determined.

通过用苯酚 - 甲酚混合物提取组织来制备核糖体RNA，并且核糖体RNA可以用间甲酚选择性沉淀。没有快速标记的RNA与这种物质相关联。2. 然而，如果用对氨基水杨酸盐和苯酚 - 甲酚混合物提取RNA和DNA并沉淀核酸，DNA、糖原和s - RNA（转移RNA）可以用3m醋酸钠提取，在这种情况下，快速标记的RNA仍然与核糖体RNA相关联。3. 核糖体RNA在浓盐溶液存在下是稳定的，尽管在10mm醋酸钠中于70度加热会使其二级结构丧失，但在200mm醋酸钠存在下它可以重新形成。4. 28s和18s组分已被分离并测定了它们的碱基组成。