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亚硫酸氢盐在链球菌抗烟酰胺腺嘌呤二核苷酸酶试验中的应用。

Use of bisulfite in the streptococcal anti-nicotinamide adenine dinucleotidase test.

作者信息

Ayoub E M, Ferretti J J

出版信息

Appl Microbiol. 1966 May;14(3):391-3. doi: 10.1128/am.14.3.391-393.1966.

DOI:10.1128/am.14.3.391-393.1966
PMID:4291236
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC546724/
Abstract

A major drawback in the performance of the streptococcal anti-nicotinamide adenine dinucleotidase test on a large scale has been the hazard involved in the use of cyanide in assaying nicotinamide adenine dinucleotide. The use of bisulfite instead of cyanide in the performance of this test was investigated. The assays obtained with bisulfite parallel closely those obtained with cyanide, and justify the replacement of cyanide by the safer bisulfite reagent.

摘要

大规模进行链球菌抗烟酰胺腺嘌呤二核苷酸酶试验时，一个主要缺点是在测定烟酰胺腺嘌呤二核苷酸时使用氰化物存在风险。研究了在该试验中使用亚硫酸氢盐代替氰化物的情况。用亚硫酸氢盐进行的测定与用氰化物进行的测定结果非常接近，证明可用更安全的亚硫酸氢盐试剂替代氰化物。

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引用本文的文献

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本文引用的文献

1
The reaction of pyridine nucleotide with cyanide and its analytical use.吡啶核苷酸与氰化物的反应及其分析应用。
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2
A FACTOR OTHER THAN STREPTOCOCCAL NICOTINAMIDE ADENINE DINUCLEOTIDASE WHICH COMBINES WITH ANTIBODY TO THIS ENZYME: ITS PRODUCTION AND EFFECT ON ANTIBODY DETERMINATIONS.一种不同于链球菌烟酰胺腺嘌呤二核苷酸酶的因子，它能与该酶的抗体结合：其产生及对抗体测定的影响。
J Immunol. 1963 May;90:793-803.
3
Serum inhibition of streptococcal diphosphopyridine nucleotidase in uncomplicated streptococcal pharyngitis and in rheumatic fever.单纯性链球菌性咽炎和风湿热中血清对链球菌二磷酸吡啶核苷酸酶的抑制作用
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Chemical properties of 3-substituted pyridine analogues of diphosphopyridine nucleotide.二磷酸吡啶核苷酸的3-取代吡啶类似物的化学性质
J Biol Chem. 1959 May;234(5):1219-25.
5
Neutralizing antibodies to streptococcal diphosphopyridine nucleotidase in the serum of experimental animals and human beings.实验动物和人类血清中针对链球菌二磷酸吡啶核苷酸酶的中和抗体。
J Exp Med. 1958 Sep 1;108(3):299-309. doi: 10.1084/jem.108.3.299.