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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Biochem J. 1967 Feb;102(2):401-6. doi: 10.1042/bj1020401.

Partially purified sucrose phosphatase from immature stem tissue of sugarcane is inhibited by sucrose. The enzyme was also inhibited by maltose, melezitose and 6-kestose but not by eight other sugars, including glucose and fructose. 2. The relative effectiveness of sucrose, maltose and melezitose as inhibitors is different for sucrose phosphatase from different plants. 3. The inhibition of the sugar-cane enzyme by sucrose was shown to be partially competitive. The K(i) for sucrose is about 10mm. 4. Melezitose is also a partially competitive inhibitor of the enzyme but the inhibition by maltose is probably mixed. 5. The possibility that sucrose controls both the rate of accumulation of sucrose in stems of sugar-cane and sucrose synthesis in leaves by inhibiting sucrose phosphatase is discussed.

从甘蔗未成熟茎组织中部分纯化得到的蔗糖磷酸酶受到蔗糖的抑制。该酶也受到麦芽糖、松三糖和潘糖的抑制，但不受包括葡萄糖和果糖在内的其他八种糖的抑制。2. 蔗糖、麦芽糖和松三糖作为抑制剂的相对效力因不同植物的蔗糖磷酸酶而异。3. 已表明蔗糖对甘蔗酶的抑制作用具有部分竞争性。蔗糖的抑制常数（Ki）约为10 mM。4. 松三糖也是该酶的部分竞争性抑制剂，但麦芽糖的抑制作用可能是混合型的。5. 讨论了蔗糖通过抑制蔗糖磷酸酶来控制甘蔗茎中蔗糖积累速率和叶片中蔗糖合成的可能性。

Biochem J. 1967 Feb;102(2):401-6. doi: 10.1042/bj1020401.

Partially purified sucrose phosphatase from immature stem tissue of sugarcane is inhibited by sucrose. The enzyme was also inhibited by maltose, melezitose and 6-kestose but not by eight other sugars, including glucose and fructose. 2. The relative effectiveness of sucrose, maltose and melezitose as inhibitors is different for sucrose phosphatase from different plants. 3. The inhibition of the sugar-cane enzyme by sucrose was shown to be partially competitive. The K(i) for sucrose is about 10mm. 4. Melezitose is also a partially competitive inhibitor of the enzyme but the inhibition by maltose is probably mixed. 5. The possibility that sucrose controls both the rate of accumulation of sucrose in stems of sugar-cane and sucrose synthesis in leaves by inhibiting sucrose phosphatase is discussed.

从甘蔗未成熟茎组织中部分纯化得到的蔗糖磷酸酶受到蔗糖的抑制。该酶也受到麦芽糖、松三糖和潘糖的抑制，但不受包括葡萄糖和果糖在内的其他八种糖的抑制。2. 蔗糖、麦芽糖和松三糖作为抑制剂的相对效力因不同植物的蔗糖磷酸酶而异。3. 已表明蔗糖对甘蔗酶的抑制作用具有部分竞争性。蔗糖的抑制常数（Ki）约为10 mM。4. 松三糖也是该酶的部分竞争性抑制剂，但麦芽糖的抑制作用可能是混合型的。5. 讨论了蔗糖通过抑制蔗糖磷酸酶来控制甘蔗茎中蔗糖积累速率和叶片中蔗糖合成的可能性。