白色肌肉和红色肌肉中果糖1,6 - 二磷酸酶、磷酸果糖激酶和磷酸烯醇式丙酮酸羧激酶的活性。

The activities of fructose 1,6-diphosphatase, phosphofructokinase and phosphoenolpyruvate carboxykinase in white muscle and red muscle.

作者信息

Opie L H, Newsholme E A

出版信息

Biochem J. 1967 May;103(2):391-9. doi: 10.1042/bj1030391.

DOI:10.1042/bj1030391

PMID:4291786

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1270420/

Abstract

The activities of fructose 1,6-diphosphatase were measured in extracts of muscles of various physiological function, and compared with the activities of other enzymes including phosphofructokinase, phosphoenolpyruvate carboxykinase and the lactate-dehydrogenase isoenzymes. 2. The activity of phosphofructokinase greatly exceeded that of fructose diphosphatase in all muscles tested, and it is concluded that fructose diphosphatase could not play any significant role in the regulation of fructose 6-phosphate phosphorylation in muscle. 3. Fructose-diphosphatase activity was highest in white muscle and low in red muscle. No activity was detected in heart or a deep-red skeletal muscle, rabbit semitendinosus. 4. The lactate-dehydrogenase isoenzyme ratio (activities at high and low substrate concentration) was measured in various muscles because a low ratio is characteristic of muscles that are more dependent on glycolysis for their energy production. As the ratio decreased the activity of fructose diphosphatase increased, which suggests that highest fructose-diphosphatase activity is found in muscles that depend most on glycolysis. 5. There was a good correlation between the activities of fructose diphosphatase and phosphoenolpyruvate carboxykinase in white muscle, where the activities of these enzymes were similar to those of liver and kidney cortex. However, the activities of pyruvate carboxylase and glucose 6-phosphatase were very low in white muscle, thereby excluding the possibility of gluconeogenesis from pyruvate and lactate. 6. It is suggested that the presence of fructose diphosphatase and phosphoenolpyruvate carboxykinase in white muscle may be related to operation of the alpha-glycerophosphate-dihydroxyacetone phosphate and malate-oxaloacetate cycles in this tissue.

摘要

测定了具有不同生理功能的肌肉提取物中1,6 - 二磷酸果糖酶的活性，并与包括磷酸果糖激酶、磷酸烯醇式丙酮酸羧激酶和乳酸脱氢酶同工酶在内的其他酶的活性进行了比较。2. 在所有测试的肌肉中，磷酸果糖激酶的活性大大超过1,6 - 二磷酸果糖酶的活性，由此得出结论，1,6 - 二磷酸果糖酶在肌肉中6 - 磷酸果糖磷酸化的调节中不可能发挥任何重要作用。3. 1,6 - 二磷酸果糖酶活性在白肌中最高，在红肌中较低。在心脏或深红色骨骼肌（兔半腱肌）中未检测到活性。4. 测定了不同肌肉中乳酸脱氢酶同工酶比率（高底物浓度和低底物浓度下的活性），因为低比率是更依赖糖酵解产生能量的肌肉的特征。随着比率降低，1,6 - 二磷酸果糖酶的活性增加，这表明在最依赖糖酵解的肌肉中发现了最高的1,6 - 二磷酸果糖酶活性。5. 在白肌中，1,6 - 二磷酸果糖酶和磷酸烯醇式丙酮酸羧激酶的活性之间存在良好的相关性，这些酶在白肌中的活性与肝脏和肾皮质中的相似。然而，丙酮酸羧化酶和葡萄糖6 - 磷酸酶在白肌中的活性非常低，从而排除了从丙酮酸和乳酸进行糖异生的可能性。6. 有人提出，白肌中1,6 - 二磷酸果糖酶和磷酸烯醇式丙酮酸羧激酶的存在可能与该组织中α - 甘油磷酸 - 磷酸二羟丙酮循环和苹果酸 - 草酰乙酸循环的运行有关。