在仓鼠血清和腹水中针对多种病毒诱导的肿瘤抗原产生高滴度抗体。
Production of high-titer antibody in serum and ascitic fluid of hamsters for a variety of virus-induced tumor antigens.
作者信息
Gilden R V, Beddow T G, Huebner R J
出版信息
Appl Microbiol. 1967 May;15(3):657-60. doi: 10.1128/am.15.3.657-660.1967.
Abstract
Methods not involving the use of viable tumors were developed for production of high-titer antibodies. The methods involved use of tumor homogenates. Homogenates from all tumor lines tested contained the characteristic tumor antigen. Attempts to isolate virus failed, and virus antigens could not be demonstrated. Immunization with tumor homogenates, in Freund's complete adjuvant, resulted in high antibody levels. None of the animals so immunized developed tumors.
摘要
为了生产高滴度抗体，研发了不涉及使用活肿瘤的方法。这些方法包括使用肿瘤匀浆。所有测试肿瘤系的匀浆都含有特征性肿瘤抗原。分离病毒的尝试失败了，且无法证明病毒抗原的存在。在弗氏完全佐剂中用肿瘤匀浆进行免疫，可产生高抗体水平。如此免疫的动物均未发生肿瘤。
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