Moss C W, Schekter M A, Cherry W B
Appl Microbiol. 1967 Jul;15(4):718-22. doi: 10.1128/am.15.4.718-722.1967.
A survey was made to determine the distribution of the enzyme neuraminidase among 76 strains of Clostridium perfringens. Representative strains from each toxigenic type (A to F) and atypical C. perfringens type A food-poisoning strains of both American and English (Hobbs types) origin were tested. Both the American food-poisoning and nonfood-poisoning associated cultures consisted of both neuraminidase-positive and -negative strains. Furthermore, American strains which could not be differentiated from the original Hobbs cultures consisted of both neuraminidase-positive and -negative representatives. In contrast, the English (Hobbs) strains uniformly failed to produce an active intracellular or extracellular neuraminidase. No enzyme activity was detected in these strains when cultures were grown in different growth media, when grown in the presence of substrate (neuraminlactose), or upon extended incubation of enzyme preparations with substrate. With the exception of a type F strain, representative strains of the other toxigenic types (A to F) produced neuraminidase; 85% of the typical type A strains contained the enzyme.
开展了一项调查，以确定神经氨酸酶在76株产气荚膜梭菌中的分布情况。对每种产毒类型（A至F）的代表性菌株以及源自美国和英国（霍布斯型）的非典型产气荚膜梭菌A型食物中毒菌株进行了检测。美国的食物中毒相关培养物和非食物中毒相关培养物均包含神经氨酸酶阳性和阴性菌株。此外，无法与原始霍布斯培养物区分的美国菌株包含神经氨酸酶阳性和阴性代表菌株。相比之下，英国（霍布斯）菌株均未能产生有活性的细胞内或细胞外神经氨酸酶。当培养物在不同生长培养基中生长时、在底物（神经氨酸乳糖）存在下生长时，或酶制剂与底物长时间孵育时，在这些菌株中均未检测到酶活性。除F型菌株外，其他产毒类型（A至F）的代表性菌株均产生神经氨酸酶；85%的典型A型菌株含有该酶。
