胚胎鸡肝脏微粒体的磷脂组成。
The phospholipid composition of embryonic chick liver microsomes.
作者信息
Ward D B, Pollak J K
出版信息
Biochem J. 1967 Sep;104(3):861-5. doi: 10.1042/bj1040861.
Abstract
- The phospholipid composition of hepatic microsomal fractions from different developmental stages of embryonic chick was established. The major components were phosphatidylcholine (approx. 66%), phosphatidylethanolamine plus phosphatidylserine (approx. 21%) and sphingomyelin (approx. 9%). 2. There were no significant changes in the phospholipid composition during embryonic development from 9 to 20 days. 3. When microsomal subfractions were prepared it was found that the smooth-microsomal fractions (Ia and Ib) had a significantly greater sphingomyelin content than the rough-microsomal fraction (II). This was compensated by a lower phosphatidylcholine content in fractions Ia and Ib and an increase of phosphatidylcholine in fraction II. 4. The significance of the differences in the phospholipid composition of smooth and rough microsomes is discussed with particular reference to the origin and interrelation of smooth and rough endoplasmic reticulum.
摘要
- 确定了来自胚胎期雏鸡不同发育阶段的肝微粒体组分的磷脂组成。主要成分是磷脂酰胆碱（约66%）、磷脂酰乙醇胺加磷脂酰丝氨酸（约21%）和鞘磷脂（约9%）。2. 在胚胎发育的9至20天期间，磷脂组成没有显著变化。3. 当制备微粒体亚组分时，发现光滑微粒体组分（Ia和Ib）的鞘磷脂含量明显高于粗糙微粒体组分（II）。这通过Ia和Ib组分中较低的磷脂酰胆碱含量以及II组分中磷脂酰胆碱的增加得到补偿。4. 特别参照光滑内质网和粗糙内质网的起源及相互关系，讨论了光滑微粒体和粗糙微粒体磷脂组成差异的意义。
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