用于从猪组织中分离鸟分枝杆菌复合群的四种培养基的比较。
Comparison of four culture media for isolation of Mycobacterium avium complex from porcine tissues.
作者信息
Thoen C O, Himes E M, Jarnagin J L, Harrington R
出版信息
J Clin Microbiol. 1979 Feb;9(2):194-6. doi: 10.1128/jcm.9.2.194-196.1979.
The efficiency of four culture media was compared for the isolation of Mycobacterium avium complex from 197 procine tissues. In 82 tissues with microscopic granulomas and acid-fast bacilli, a significantly greater number of isolates were obtained on Middlebrook 7H10 medium with sodium pyruvate than on Stonebrink medium, Herrold egg yolk agar medium, or Lowenstein-Jensen medium (P=0.01). In 46 tissues in which no microscopic granulomas or acid-fast bacilli were observed, a significantly greater number of isolates were made on Middlebrook 7H10 medium or Herrold egg yolk agar medium than on Stonebrink medium or on Lowenstein-Jensen medium (P=0.01). The time required to grow M. avium complex on Lowenstein-Jensen medium was significantly greater than the time required to observe growth on Stonebrink, Middlebrook 7H10, or Herrold egg yolk agar medium (p=0.001).
比较了四种培养基从197份猪组织中分离鸟分枝杆菌复合群的效率。在82份有显微镜下肉芽肿和抗酸杆菌的组织中，添加丙酮酸钠的Middlebrook 7H10培养基上获得的分离株数量显著多于Stonebrink培养基、Herrold蛋黄琼脂培养基或Lowenstein-Jensen培养基（P=0.01）。在46份未观察到显微镜下肉芽肿或抗酸杆菌的组织中，Middlebrook 7H10培养基或Herrold蛋黄琼脂培养基上获得的分离株数量显著多于Stonebrink培养基或Lowenstein-Jensen培养基（P=0.01）。在Lowenstein-Jensen培养基上培养鸟分枝杆菌复合群所需的时间显著长于在Stonebrink、Middlebrook 7H10或Herrold蛋黄琼脂培养基上观察到生长所需的时间（p=0.001）。
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