Hunter W M, Bennie J G
J Endocrinol. 1979 Jan;80(1):59-68. doi: 10.1677/joe.0.0800059.
Serum and plasma from human and domestic animals contain variable amounts of non-specific material(s) which may be mistaken for hormone in assays for human LH and FSH, based upon antisera of high sensitivity and hormonal monospecificity. The non-specific response curves are generally, but not invariably, less steep than those of the hormone standards and endogenous homologous hormones. The levels of non-specific intrusion can be of sufficient magnitude to obscure specific estimations seriously, particularly at low hormone levels, unless the assays are designed to minimize this effect. The non-specific effects could be minimized (but not abolished) by careful optimization of the assays which involved making the response curve as sensitive as possible and incorporating the serum at a final dilution of 1 : 2, since further dilution increased the relative contribution of the non-specific substance(s). The optimized assays require only 48 h of total incubation and show a sevenfold increase in the mean concentration of LH between sera from prepubertal children and adults accompanied by a mean threefold difference in the concentration of FSH.
人和家畜的血清及血浆中含有数量不等的非特异性物质，在基于高灵敏度和激素单特异性抗血清的人促黄体生成素（LH）和促卵泡生成素（FSH）检测中，这些物质可能会被误认为是激素。非特异性反应曲线通常（但并非总是）比激素标准品和内源性同源激素的反应曲线平缓。非特异性干扰水平可能足够高，严重影响特异性检测结果，尤其是在激素水平较低时，除非检测方法经过设计以尽量减小这种影响。通过仔细优化检测方法可将非特异性影响降至最低（但无法消除），具体做法包括使反应曲线尽可能灵敏，并以1:2的终末稀释度加入血清，因为进一步稀释会增加非特异性物质的相对贡献。优化后的检测方法总共只需孵育48小时，青春期前儿童和成年人血清中LH的平均浓度增加了7倍，同时FSH浓度平均相差3倍。
