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[L细胞培养中细胞间交换强度的变化]

[Changes in intercellular exchange intensity in an L cell culture].

作者信息

Potapova T V, Chailakhian L M

出版信息

Tsitologiia. 1979 Nov;21(11):1289-95.

PMID:43000
Abstract

Intercellular junctions permeable to fluorescein Na were studied with the aid of intracellular glass microelectrodes in the cultures of transformed mouse-embryo cells (L-strain). The degree of coupling was correlated with the cell culture density. In addition, the degree of coupling increased after short-time incubated of the cells with 1mM lanthanum. Such an incubation did not influence other features of cells: the value of the membrane potential, the character of growth or the inclusion of the vital dye neutral red. Some other factors stimulated cellular aggregation (concanavalin A, protamine, decreasing the pH) but did not influence the degree of coupling.

摘要

借助细胞内玻璃微电极,在转化的小鼠胚胎细胞(L细胞系)培养物中研究了对荧光素钠具有通透性的细胞间连接。偶联程度与细胞培养密度相关。此外,用1mM镧对细胞进行短时间孵育后,偶联程度增加。这种孵育不影响细胞的其他特征:膜电位值、生长特性或活体染料中性红的摄入。其他一些因素可刺激细胞聚集(伴刀豆球蛋白A、鱼精蛋白、降低pH值),但不影响偶联程度。

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