Shieh T R, Ware J H
Appl Microbiol. 1968 Sep;16(9):1348-51. doi: 10.1128/am.16.9.1348-1351.1968.
A culture enrichment technique was used to isolate phytase-producing microorganisms. Also, microorganisms from various culture collections were tested for their phytase-producing ability. A number of the Aspergillus niger group produced extracellular phytase which dephosphorylated calcium phytate in acidic solution. A soil isolate, A. ficuum NRRL 3135, produced the most active phytase in a cornstarch-based medium. Production of phytase was strongly repressed by inorganic phosphates and required a high carbon to phosphorus ratio in the medium.
采用一种培养富集技术来分离产植酸酶的微生物。此外,还对来自各种培养物保藏中心的微生物进行了产植酸酶能力的测试。许多黑曲霉属的菌株在酸性溶液中产生能使植酸钙脱磷酸的胞外植酸酶。从土壤中分离得到的烟曲霉NRRL 3135在以玉米淀粉为基础的培养基中产生的植酸酶活性最高。植酸酶的产生受到无机磷酸盐的强烈抑制,并且培养基中需要高碳磷比。