Ullah A H
Southern Regional Research Laboratory, ARS, USDA, New Orleans, Louisiana 70124.
Prep Biochem. 1988;18(4):443-58. doi: 10.1080/00327488808062543.
A rapid purification scheme utilizing three chromatographic steps resulted in 6 fold purification of Aspergillus ficuum phytase (myo-inositol-hexakisphosphate 3-phosphohydrolase, EC 3.1.3.8). At pH 5.0 and 60 degrees C the enzyme performed acceptably for 2.0 hr with only 30% diminished catalytic rate at the end. Substrate concentration exceeding 2mM was inhibitory. The inorganic orthophosphate, the product and a weak inhibitor, exhibited a Ki of 1.9 x 10(-3)M. The extracellular phytase has the potential for industrial use since it can be over produced, easily purified, remain catalytically active for a longer period and is not subjected to severe product inhibition.
一种利用三步色谱法的快速纯化方案实现了米曲霉植酸酶（肌醇六磷酸3 - 磷酸水解酶，EC 3.1.3.8）6倍的纯化。在pH 5.0和60℃条件下，该酶在2.0小时内表现良好，最终催化速率仅降低30%。底物浓度超过2mM具有抑制作用。无机正磷酸盐作为产物和弱抑制剂，其抑制常数Ki为1.9×10⁻³M。这种胞外植酸酶具有工业应用潜力，因为它可以过量生产、易于纯化、能在较长时间内保持催化活性且不受严重的产物抑制。
