真菌植酸酶：从基因到应用。
Fungal phytases: from genes to applications.
机构信息
Department of Microbiology/BIOAGRO, Federal University of Viçosa, Av. Peter Henry Rolfs s/n, Vicosa, MG, 36570-000, Brazil.
出版信息
Braz J Microbiol. 2020 Sep;51(3):1009-1020. doi: 10.1007/s42770-020-00289-y. Epub 2020 May 14.
Abstract
Phytic acid stores 60-90% of the inorganic phosphorus in legumes, oil seeds, and cereals, making it inaccessible for metabolic processes in living systems. In addition, given its negative charge, phytic acid complexes with divalent cations, starch, and proteins. Inorganic phosphorous can be released from phytic acid upon the action of phytases. Phytases are phosphatases produced by animals, plants, and microorganisms, notably Aspergillus niger, and are employed as animal feed additive, in chemical industry and for ethanol production. Given the industrial relevance of phytases produced by filamentous fungi, this work discusses the functional characterization of fungal phytase-coding genes/proteins, highlighting the physicochemical parameters that govern the enzymatic activity, the development of phytase super-producing strains, and key features for industrial applications.
摘要
植酸在豆类、油籽和谷物中储存了 60-90%的无机磷，使其无法被生物体内的代谢过程利用。此外，由于其带负电荷，植酸与二价阳离子、淀粉和蛋白质形成复合物。植酸在植酸酶的作用下可以释放出无机磷。植酸酶是动物、植物和微生物（尤其是黑曲霉）产生的磷酸酶，被用作动物饲料添加剂、在化学工业和乙醇生产中。鉴于丝状真菌产生的植酸酶具有工业相关性，这项工作讨论了真菌植酸酶编码基因/蛋白的功能特征，重点介绍了控制酶活性的理化参数、植酸超产生菌株的开发以及工业应用的关键特征。
相似文献
1
Fungal phytases: from genes to applications.真菌植酸酶：从基因到应用。
Braz J Microbiol. 2020 Sep;51(3):1009-1020. doi: 10.1007/s42770-020-00289-y. Epub 2020 May 14.
2
Replacement P212H altered the pH-temperature profile of phytase from Aspergillus niger NII 08121.替换P212H改变了黑曲霉NII 08121植酸酶的pH-温度曲线。
Appl Biochem Biotechnol. 2015 Mar;175(6):3084-92. doi: 10.1007/s12010-015-1485-6. Epub 2015 Jan 17.
3
Biochemical characterization of fungal phytases (myo-inositol hexakisphosphate phosphohydrolases): catalytic properties.真菌植酸酶（肌醇六磷酸磷酸水解酶）的生化特性：催化特性
Appl Environ Microbiol. 1999 Feb;65(2):367-73. doi: 10.1128/AEM.65.2.367-373.1999.
4
Enhancement of thermostability and kinetic efficiency of Aspergillus niger PhyA phytase by site-directed mutagenesis.通过定点诱变提高黑曲霉PhyA植酸酶的热稳定性和动力学效率。
Appl Biochem Biotechnol. 2015 Mar;175(5):2528-41. doi: 10.1007/s12010-014-1440-y. Epub 2014 Dec 20.
5
Improved thermostability and enzyme activity of a recombinant phyA mutant phytase from Aspergillus niger N25 by directed evolution and site-directed mutagenesis.通过定向进化和定点突变提高黑曲霉 N25 重组 phyA 突变植酸酶的热稳定性和酶活性。
Enzyme Microb Technol. 2018 Jan;108:74-81. doi: 10.1016/j.enzmictec.2017.09.010. Epub 2017 Sep 23.
6
Biophysical characterization of fungal phytases (myo-inositol hexakisphosphate phosphohydrolases): molecular size, glycosylation pattern, and engineering of proteolytic resistance.真菌植酸酶（肌醇六磷酸磷酸水解酶）的生物物理特性：分子大小、糖基化模式及抗蛋白酶工程
Appl Environ Microbiol. 1999 Feb;65(2):359-66. doi: 10.1128/AEM.65.2.359-366.1999.
7
Shifting the pH profile of Aspergillus niger PhyA phytase to match the stomach pH enhances its effectiveness as an animal feed additive.改变黑曲霉植酸酶A的pH分布以匹配胃内pH值，可提高其作为动物饲料添加剂的有效性。
Appl Environ Microbiol. 2006 Jun;72(6):4397-403. doi: 10.1128/AEM.02612-05.
8
Gene cloning and soluble expression of Aspergillus niger phytase in E. coli cytosol via chaperone co-expression.通过共表达伴侣蛋白在大肠杆菌胞质溶胶中克隆黑曲霉植酸酶基因并进行可溶性表达。
Biotechnol Lett. 2014 Jan;36(1):85-91. doi: 10.1007/s10529-013-1322-3.
9
Expression and characterization of Aspergillus thermostable phytases in Pichia pastoris.嗜热栖热放线菌植酸酶在毕赤酵母中的表达与鉴定
FEMS Microbiol Lett. 2009 Jan;290(1):18-24. doi: 10.1111/j.1574-6968.2008.01399.x. Epub 2008 Nov 12.
10
Biofactories for the Production of Recombinant Phytases and their Application in the Animal Feed Industry.用于生产重组植酸酶的生物工厂及其在动物饲料工业中的应用。
Recent Pat Biotechnol. 2018;12(2):113-125. doi: 10.2174/1872208311666170915161848.
引用本文的文献
1
Assessment of Recombinant β-Propeller Phytase of the Species Expressed Intracellularly in .在[具体物种]中细胞内表达的该物种重组β-螺旋桨植酸酶的评估。 （原文中“in.”后面缺少具体信息，翻译时保留原文形式）
J Fungi (Basel). 2025 Feb 26;11(3):186. doi: 10.3390/jof11030186.
2
Phosphorus dynamics and sustainable agriculture: The role of microbial solubilization and innovations in nutrient management.磷动态与可持续农业：微生物溶解作用及养分管理创新的作用
Curr Res Microb Sci. 2024 Nov 27;8:100326. doi: 10.1016/j.crmicr.2024.100326. eCollection 2025.
3
Bioinformatics study of phytase from Aspergillus niger for use as feed additive in livestock feed.黑曲霉植酸酶用作家畜饲料添加剂的生物信息学研究
J Genet Eng Biotechnol. 2023 Nov 27;21(1):142. doi: 10.1186/s43141-023-00600-y.
4
Microbial Phytases: Properties and Applications in the Food Industry.微生物植酸酶：性质及其在食品工业中的应用。
Curr Microbiol. 2023 Oct 17;80(12):374. doi: 10.1007/s00284-023-03471-1.
5
A Comprehensive Insight into Fungal Enzymes: Structure, Classification, and Their Role in Mankind's Challenges.对真菌酶的全面洞察：结构、分类及其在人类面临挑战中的作用
J Fungi (Basel). 2021 Dec 28;8(1):23. doi: 10.3390/jof8010023.
6
Effects of Phytic Acid-Degrading Bacteria on Mineral Element Content in Mice.植酸降解菌对小鼠体内矿物质元素含量的影响。
Front Microbiol. 2021 Nov 22;12:753195. doi: 10.3389/fmicb.2021.753195. eCollection 2021.
7
Secretome Analysis of - Interaction Unveils New Roles for the Plant Glutamate:Glyoxylate Aminotransferase GGAT1 in Plant Growth Induced by the Fungus and Resistance against .- 互作的分泌组分析揭示了植物谷氨酸：乙醛酸转氨酶 GGAT1 在真菌诱导的植物生长和对 的抗性中的新作用。
Int J Mol Sci. 2021 Jun 24;22(13):6804. doi: 10.3390/ijms22136804.
本文引用的文献
1
The pH Signaling Transcription Factor PAC-3 Regulates Metabolic and Developmental Processes in Pathogenic Fungi.pH信号转录因子PAC-3调节致病真菌的代谢和发育过程。
Front Microbiol. 2019 Sep 4;10:2076. doi: 10.3389/fmicb.2019.02076. eCollection 2019.
2
Probiotic expressing heterologous phytase improves the immune system and attenuates inflammatory response in zebrafish fed with a diet rich in soybean meal.表达异源植酸酶的益生菌可改善富含豆粕日粮饲养的斑马鱼的免疫系统并减轻炎症反应。
Fish Shellfish Immunol. 2019 Oct;93:652-658. doi: 10.1016/j.fsi.2019.08.030. Epub 2019 Aug 11.
3
Production of Fungal Phytases from Agroindustrial Byproducts for Pig Diets.利用农业副产物生产真菌植酸酶用于猪饲料。
Sci Rep. 2019 Jun 25;9(1):9256. doi: 10.1038/s41598-019-45720-z.
4
Functional Metagenomics Reveals a New Catalytic Domain, the Metallo-β-Lactamase Superfamily Domain, Associated with Phytase Activity.功能宏基因组学揭示了一种新的催化结构域，即金属-β-内酰胺酶超家族结构域，与植酸酶活性有关。
mSphere. 2019 Jun 19;4(3):e00167-19. doi: 10.1128/mSphere.00167-19.
5
Genetic transformation of tropical maize ( L.) inbred line with a phytase gene from .用来自[具体来源未给出]的植酸酶基因对热带玉米（L.）自交系进行遗传转化。
3 Biotech. 2019 Jun;9(6):208. doi: 10.1007/s13205-019-1731-7. Epub 2019 May 9.
6
Thermostable phytase in feed and fuel industries.饲料和燃料工业中的耐热植酸酶。
Bioresour Technol. 2019 Apr;278:400-407. doi: 10.1016/j.biortech.2019.01.065. Epub 2019 Jan 18.
7
Enhancing thermal tolerance of Aspergillus niger PhyA phytase directed by structural comparison and computational simulation.通过结构比较和计算模拟指导黑曲霉 PhyA 植酸酶提高耐热性。
BMC Biotechnol. 2018 Jun 1;18(1):36. doi: 10.1186/s12896-018-0445-y.
8
Novel transgenic pigs with enhanced growth and reduced environmental impact.具有增强生长和降低环境影响的新型转基因猪。
Elife. 2018 May 22;7:e34286. doi: 10.7554/eLife.34286.
9
Xylanase, protease and superdosing phytase interactions in broiler performance, carcass yield and digesta transit time.木聚糖酶、蛋白酶和超量植酸酶对肉鸡生产性能、胴体产量及食糜通过时间的相互作用
Anim Nutr. 2017 Jun;3(2):121-126. doi: 10.1016/j.aninu.2017.02.001. Epub 2017 Feb 10.
10
Recent advances in enhanced enzyme activity, thermostability and secretion by N-glycosylation regulation in yeast.酵母中通过N-糖基化调控提高酶活性、热稳定性及分泌的研究进展
Biotechnol Lett. 2018 May;40(5):847-854. doi: 10.1007/s10529-018-2526-3. Epub 2018 Feb 15.
Zotero 插件