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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Fraser D R, Kodicek E

Biochem J. 1968 Sep;109(3):457-67. doi: 10.1042/bj1090457.

The mechanism of vitamin D esterification in the rat was studied with liver, small-intestinal mucosa, pancreatic juice and blood plasma as enzyme sources and [1-(3)H]cholecalciferol, [U-(14)C]ergocalciferol and [4-(14)C]cholesterol as substrates. 2. No esterification of vitamin D could be detected with liver preparations nor with homogenates or acetone-dried powder extracts of intestinal mucosa. 3. Pancreatic juice esterified [1-(3)H]cholecalciferol with oleic acid, and specificity studies indicated that a cholesterol-esterifying enzyme was using vitamin D as substrate. 4. Plasma cholesterol-esterifying enzyme also esterified vitamin D. 5. The specificity of the esterification reaction is discussed in relation to (a) the molecular structure of the substrates and (b) their availability, in a micellar solution, to the enzyme. 6. It is concluded that cholesterol-esterifying enzymes esterify vitamin D in vivo during absorption from the small intestine and while it is transported in blood.

以肝脏、小肠黏膜、胰液和血浆作为酶源，以[1-(3)H]胆钙化醇、[U-(14)C]麦角钙化醇和[4-(14)C]胆固醇作为底物，研究了大鼠体内维生素D酯化的机制。2. 用肝脏制剂以及小肠黏膜匀浆或丙酮干粉提取物均未检测到维生素D的酯化。3. 胰液可使[1-(3)H]胆钙化醇与油酸发生酯化，特异性研究表明一种胆固醇酯化酶将维生素D用作底物。4. 血浆胆固醇酯化酶也可使维生素D酯化。5. 结合以下两方面讨论了酯化反应的特异性：(a) 底物的分子结构；(b) 在胶束溶液中底物对酶的可及性。6. 得出结论：胆固醇酯化酶在维生素D从小肠吸收以及在血液中运输的过程中使其在体内发生酯化。

Fraser D R, Kodicek E

Biochem J. 1968 Sep;109(3):457-67. doi: 10.1042/bj1090457.

The mechanism of vitamin D esterification in the rat was studied with liver, small-intestinal mucosa, pancreatic juice and blood plasma as enzyme sources and [1-(3)H]cholecalciferol, [U-(14)C]ergocalciferol and [4-(14)C]cholesterol as substrates. 2. No esterification of vitamin D could be detected with liver preparations nor with homogenates or acetone-dried powder extracts of intestinal mucosa. 3. Pancreatic juice esterified [1-(3)H]cholecalciferol with oleic acid, and specificity studies indicated that a cholesterol-esterifying enzyme was using vitamin D as substrate. 4. Plasma cholesterol-esterifying enzyme also esterified vitamin D. 5. The specificity of the esterification reaction is discussed in relation to (a) the molecular structure of the substrates and (b) their availability, in a micellar solution, to the enzyme. 6. It is concluded that cholesterol-esterifying enzymes esterify vitamin D in vivo during absorption from the small intestine and while it is transported in blood.

以肝脏、小肠黏膜、胰液和血浆作为酶源，以[1-(3)H]胆钙化醇、[U-(14)C]麦角钙化醇和[4-(14)C]胆固醇作为底物，研究了大鼠体内维生素D酯化的机制。2. 用肝脏制剂以及小肠黏膜匀浆或丙酮干粉提取物均未检测到维生素D的酯化。3. 胰液可使[1-(3)H]胆钙化醇与油酸发生酯化，特异性研究表明一种胆固醇酯化酶将维生素D用作底物。4. 血浆胆固醇酯化酶也可使维生素D酯化。5. 结合以下两方面讨论了酯化反应的特异性：(a) 底物的分子结构；(b) 在胶束溶液中底物对酶的可及性。6. 得出结论：胆固醇酯化酶在维生素D从小肠吸收以及在血液中运输的过程中使其在体内发生酯化。