大鼠胆汁中维生素D代谢产物的研究。一种主要代谢产物的分离与部分鉴定。
Investigations on metabolites of vitamin D in rat bile. Separation and partial identification of a major metabolite.
作者信息
Bell P A, Kodicek E
出版信息
Biochem J. 1969 Dec;115(4):663-9. doi: 10.1042/bj1150663.
Abstract
- Young rats with cannulated bile ducts were given 0.34mg. of [1alpha-(3)H]cholecalciferol or 0.54mg. of [(14)C]ergocalciferol by intravenous infusion. Of the radioactivity in the dose of [1alpha-(3)H]cholecalciferol 31% was recovered in bile within 24hr. 2. The metabolites in bile were separated by gradient-elution column chromatography on silicic acid into five components, all more polar than cholecalciferol or 25-hydroxycholecalciferol. [(14)C]Ergocalciferol gave a similar pattern of metabolites in bile. 3. The three most polar metabolites were shown to be ionic. The major component has been identified as a glucuronide conjugate, which was not identical with synthetic cholecalciferyl glucuronide.
摘要
- 给胆管插管的幼鼠静脉输注0.34毫克的[1α-(3)H]胆钙化醇或0.54毫克的[(14)C]麦角钙化醇。在24小时内，[1α-(3)H]胆钙化醇剂量中的放射性有31%在胆汁中回收。2. 胆汁中的代谢产物通过在硅酸上的梯度洗脱柱色谱法分离为五个组分，所有组分的极性都比胆钙化醇或25-羟基胆钙化醇大。[(14)C]麦角钙化醇在胆汁中产生类似的代谢产物模式。3. 三种极性最强的代谢产物显示为离子型。主要成分已被鉴定为葡糖醛酸苷共轭物，它与合成的胆钙化醇葡糖醛酸苷不同。
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