Nichol F R, Tershak D R
J Virol. 1968 May;2(5):415-20. doi: 10.1128/JVI.2.5.415-420.1968.
A temperature-sensitive strain of type 1 poliovirus, LSc, was functionally rescued when infected cells were incubated at 40 C in the presence of Mahoney, a temperature-resistant strain of type 1 poliovirus. The rescue value was 9% of the mutant yield obtained under permissive conditions. Rescued virus underwent replication, because the progeny of (32)P-labeled LSc were not radiosensitive. Serum inactivation studies with Mahoney specific antiserum indicated that a small amount of phenotypic mixing occurred among the rescued particles. The temperature-sensitive event occurred between 2 and 4 hr postinfection in the developmental cycle of LSc. Neither viral polymerase activity nor virus-induced ribonucleic acid could be demonstrated in infected cells between 2 and 4 hr after infection at 40 C with the temperature-sensitive mutant.
当感染细胞在40℃下与1型脊髓灰质炎病毒的温度抗性毒株马奥尼一起孵育时，1型脊髓灰质炎病毒的温度敏感毒株LSc在功能上得到了拯救。拯救值为在允许条件下获得的突变体产量的9%。拯救的病毒进行了复制，因为用（32）P标记的LSc的子代对辐射不敏感。用马奥尼特异性抗血清进行的血清灭活研究表明，在拯救的颗粒之间发生了少量的表型混合。温度敏感事件发生在LSc发育周期感染后2至4小时之间。在用温度敏感突变体在40℃感染后2至4小时内，在感染细胞中既未检测到病毒聚合酶活性，也未检测到病毒诱导的核糖核酸。
