Mikhejeva A, Yakobson E, Soloviev G Y
J Virol. 1970 Aug;6(2):188-93. doi: 10.1128/JVI.6.2.188-193.1970.
Investigation of 15 poliovirus temperature-sensitive (ts) mutants by using physiological tests [formation of virus-specific antigen and ribonucleic acid (RNA) under nonpermissive conditions] permitted us to divide them into three groups. From each group, one mutant was selected (ts 2, 5, 11), and a comparative study of poliovirus-related particle (5, 10, 73, and 150S) formation under permissive (36 C) and nonpermissive (40 C) conditions was carried out. The ts 2 and ts 11 are mutants with greatly reduced RNA synthesis which at 40 C produce particles with a sedimentation constant of 5S, and the ts 5 (RNA(+)) mutant produces both 5 and 10S particles. The relationship between different temperature-sensitive defects in the mutants is discussed. The results obtained indicate a possible role of 5S protein structures in morphogenesis of poliovirus.
通过生理测试（在非允许条件下病毒特异性抗原和核糖核酸（RNA）的形成）对15种脊髓灰质炎病毒温度敏感（ts）突变体进行研究，使我们能够将它们分为三组。从每组中选择一个突变体（ts 2、5、11），并在允许温度（36℃）和非允许温度（40℃）条件下对脊髓灰质炎病毒相关颗粒（5、10、73和150S）的形成进行了比较研究。ts 2和ts 11是RNA合成大幅减少的突变体，它们在40℃时产生沉降常数为5S的颗粒，而ts 5（RNA(+)）突变体则产生5S和10S颗粒。讨论了突变体中不同温度敏感缺陷之间的关系。所得结果表明5S蛋白结构在脊髓灰质炎病毒形态发生中可能发挥的作用。
