一些脊髓灰质炎病毒温度敏感突变体的特性以及在非允许条件下脊髓灰质炎病毒相关颗粒的形成

Characterization of some poliovirus temperature-sensitive mutants and poliovirus-related particle formation under nonpermissive conditions.

作者信息

Mikhejeva A, Yakobson E, Soloviev G Y

出版信息

J Virol. 1970 Aug;6(2):188-93. doi: 10.1128/JVI.6.2.188-193.1970.

DOI:10.1128/JVI.6.2.188-193.1970

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC376107/

Abstract

Investigation of 15 poliovirus temperature-sensitive (ts) mutants by using physiological tests [formation of virus-specific antigen and ribonucleic acid (RNA) under nonpermissive conditions] permitted us to divide them into three groups. From each group, one mutant was selected (ts 2, 5, 11), and a comparative study of poliovirus-related particle (5, 10, 73, and 150S) formation under permissive (36 C) and nonpermissive (40 C) conditions was carried out. The ts 2 and ts 11 are mutants with greatly reduced RNA synthesis which at 40 C produce particles with a sedimentation constant of 5S, and the ts 5 (RNA(+)) mutant produces both 5 and 10S particles. The relationship between different temperature-sensitive defects in the mutants is discussed. The results obtained indicate a possible role of 5S protein structures in morphogenesis of poliovirus.

摘要

通过生理测试（在非允许条件下病毒特异性抗原和核糖核酸（RNA）的形成）对15种脊髓灰质炎病毒温度敏感（ts）突变体进行研究，使我们能够将它们分为三组。从每组中选择一个突变体（ts 2、5、11），并在允许温度（36℃）和非允许温度（40℃）条件下对脊髓灰质炎病毒相关颗粒（5、10、73和150S）的形成进行了比较研究。ts 2和ts 11是RNA合成大幅减少的突变体，它们在40℃时产生沉降常数为5S的颗粒，而ts 5（RNA(+)）突变体则产生5S和10S颗粒。讨论了突变体中不同温度敏感缺陷之间的关系。所得结果表明5S蛋白结构在脊髓灰质炎病毒形态发生中可能发挥的作用。

相似文献

1

Characterization of some poliovirus temperature-sensitive mutants and poliovirus-related particle formation under nonpermissive conditions.一些脊髓灰质炎病毒温度敏感突变体的特性以及在非允许条件下脊髓灰质炎病毒相关颗粒的形成

J Virol. 1970 Aug;6(2):188-93. doi: 10.1128/JVI.6.2.188-193.1970.

2

Studies on temperature-sensitive events in synthesis of poliovirus ts mutants.脊髓灰质炎病毒温度敏感突变体合成中温度敏感事件的研究。

Arch Gesamte Virusforsch. 1973;43(4):352-8. doi: 10.1007/BF01556152.

3

Synthesis of double-stranded RNA by poliovirus temperature-sensitive mutants.

Virology. 1970 Apr;40(4):971-7. doi: 10.1016/0042-6822(70)90143-1.

4

Synthesis of the virus-specific proteins of a conditionally lethal mutant of poliovirus under nonpermissive conditions.脊髓灰质炎病毒条件致死突变体在非允许条件下病毒特异性蛋白质的合成。

Virology. 1970 Mar;40(3):595-604. doi: 10.1016/0042-6822(70)90204-7.

5

[The temperature sensitive mutants of poliovirus. I. Acquisition and characteristics of the mutants].[脊髓灰质炎病毒的温度敏感突变体。I. 突变体的获得与特性]

Vopr Virusol. 1971 Jan-Feb;16(1):48-52.

6

Temperature-sensitive mutants of vesicular stomatitis virus: synthesis of virus-specific proteins.水泡性口炎病毒的温度敏感突变体：病毒特异性蛋白质的合成

J Virol. 1971 May;7(5):651-62. doi: 10.1128/JVI.7.5.651-662.1971.

7

Synthesis of cellular and viral macromolecules under conditions of suboptimal temperature.在次优温度条件下细胞和病毒大分子的合成。

Arch Gesamte Virusforsch. 1970;30(1):7-15. doi: 10.1007/BF01262577.

8

Effect of inactivation by hydroxylamine on early functions of poliovirus.羟胺灭活对脊髓灰质炎病毒早期功能的影响。

J Virol. 1971 Jul;8(1):1-6. doi: 10.1128/JVI.8.1.1-6.1971.

9

Study of the transcription and the replication of vesicular stomatitis virus by using temperature-sensitive mutants.利用温度敏感突变体研究水疱性口炎病毒的转录和复制

J Virol. 1972 Nov;10(5):889-95. doi: 10.1128/JVI.10.5.889-895.1972.

10

Proteins associated with the poliovirus RNA replication complex.与脊髓灰质炎病毒RNA复制复合体相关的蛋白质。

Virology. 1971 Nov;46(2):375-86. doi: 10.1016/0042-6822(71)90039-0.

引用本文的文献

1

Human rhinovirus type 16: mutant V1210A requires capsid-binding drug for assembly of pentamers to form virions during morphogenesis.人鼻病毒16型：突变体V1210A在形态发生过程中组装五聚体形成病毒粒子时需要衣壳结合药物。

J Virol. 2003 Jun;77(11):6235-44. doi: 10.1128/jvi.77.11.6235-6244.2003.

2

Studies on temperature-sensitive events in synthesis of poliovirus ts mutants.脊髓灰质炎病毒温度敏感突变体合成中温度敏感事件的研究。

Arch Gesamte Virusforsch. 1973;43(4):352-8. doi: 10.1007/BF01556152.

3

Correlation between TS phenotype and pathogenicity of some animal viruses.TS表型与某些动物病毒致病性之间的相关性。

Arch Gesamte Virusforsch. 1973;42(2):154-9. doi: 10.1007/BF01270835.

4

Study of some stages of poliovirus morphogenesis in MiO cells.脊髓灰质炎病毒在MiO细胞中形态发生某些阶段的研究。

J Virol. 1972 Aug;10(2):261-6. doi: 10.1128/JVI.10.2.261-266.1972.

本文引用的文献

1

Localization of antigen in tissue cells; improvements in a method for the detection of antigen by means of fluorescent antibody.组织细胞中抗原的定位；通过荧光抗体检测抗原的方法的改进。

J Exp Med. 1950 Jan 1;91(1):1-13. doi: 10.1084/jem.91.1.1.

2

Morphogenesis of poliovirus. I. Association of the viral RNA with coat protein.脊髓灰质炎病毒的形态发生。I. 病毒RNA与衣壳蛋白的结合。

J Mol Biol. 1968 Apr 28;33(2):369-78. doi: 10.1016/0022-2836(68)90195-2.

3

Polypeptide cleavages in the formation of poliovirus proteins.脊髓灰质炎病毒蛋白形成过程中的多肽裂解

Proc Natl Acad Sci U S A. 1968 Sep;61(1):77-84. doi: 10.1073/pnas.61.1.77.

4

A genetic map of poliovirus temperature-sensitive mutants.脊髓灰质炎病毒温度敏感突变体的遗传图谱。

Virology. 1968 Aug;35(4):584-96. doi: 10.1016/0042-6822(68)90287-0.

5

In vitro assembly of poliovirus-related particles.脊髓灰质炎病毒相关颗粒的体外组装。

Virology. 1968 Jun;35(2):216-26. doi: 10.1016/0042-6822(68)90262-6.

6

Specific cleavage of viral proteins as steps in the synthesis and maturation of enteroviruses.作为肠道病毒合成与成熟步骤的病毒蛋白特异性切割

Proc Natl Acad Sci U S A. 1968 Jul;60(3):1015-22. doi: 10.1073/pnas.60.3.1015.

7

Production of infectious RNA and serum-blocking antigen by poliovirus temperature-sensitive mutants.

J Gen Virol. 1968 Mar;2(2):297-307. doi: 10.1099/0022-1317-2-2-297.

8

Evidence for large precursor proteins in poliovirus synthesis.脊髓灰质炎病毒合成中存在大前体蛋白的证据。

Proc Natl Acad Sci U S A. 1968 Mar;59(3):966-71. doi: 10.1073/pnas.59.3.966.

9

Physiological characterization of heat-defective (temperature-sensitive) poliovirus mutants: preliminary classification.

Virology. 1966 Dec;30(4):638-49. doi: 10.1016/0042-6822(66)90169-3.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

文档翻译

学术文献翻译模型，支持多种主流文档格式。