Tershak D R
J Virol. 1969 Mar;3(3):297-303. doi: 10.1128/JVI.3.3.297-303.1969.
The synthesis of viral ribonucleic acid (RNA) was detected within 2 hr after infection with LSc poliovirus at 35 C. This RNA eluted as a single peak with 0.9 m NaCl on methylated albumin celite columns, was sensitive to ribonuclease, precipitated in the presence of 2 m LiCl, and had an S(20) value at 34 +/- 2 in linear sucrose gradients. When cells were infected at 39 to 40 C, there was also early synthesis of RNA. However, 2 hr after infection this synthesis was drastically inhibited. The absence of net RNA synthesis at 39 to 40 C during the late stages of infection was not caused by rapid degradation of newly formed RNA, since the RNA produced between 1 and 2 hr at 39 to 40 C was still present 3.5 hr after infection. There was a 3 log(10) inhibition in the production of infectious virus when p-fluorophenylalanine was present in the medium at a concentration of 25 mug/ml. This concentration of analogue had little effect upon the production of viral polymerase and viral RNA. Virus grown in the presence of analogue at a concentration of 10 mug/ml exhibited increased heat sensitivity compared to control virus. However, viral polymerase exhibited no change in sensitivity to heat or manganese when cells were exposed to 25 mug of p-fluorophenylalanine per ml during infection. p-Fluorophenylalanine had a relatively selective effect on viral capsid protein but did not reverse the inhibition of synthesis of viral RNA at 39 to 40 C.
在35℃用LSc脊髓灰质炎病毒感染后2小时内可检测到病毒核糖核酸（RNA）的合成。这种RNA在用0.9m NaCl在甲基化白蛋白硅藻土柱上洗脱时呈现单峰，对核糖核酸酶敏感，在2m LiCl存在下可沉淀，并且在线性蔗糖梯度中的S(20)值为34±2。当细胞在39至40℃感染时，也有早期RNA合成。然而，感染后2小时这种合成被急剧抑制。在感染后期39至40℃时净RNA合成的缺失不是由新形成的RNA快速降解引起的，因为在39至40℃ 1至2小时产生的RNA在感染后3.5小时仍然存在。当培养基中对氟苯丙氨酸浓度为25μg/ml时，传染性病毒的产生受到3个对数（10）的抑制。这种浓度的类似物对病毒聚合酶和病毒RNA的产生影响很小。与对照病毒相比，在10μg/ml浓度的类似物存在下生长的病毒表现出对热敏感性增加。然而，当细胞在感染期间暴露于每毫升25μg对氟苯丙氨酸时，病毒聚合酶对热或锰的敏感性没有变化。对氟苯丙氨酸对病毒衣壳蛋白有相对选择性的作用，但不能逆转在39至40℃时病毒RNA合成的抑制。
