Aaronson S A, Todaro G J
Science. 1968 Nov 29;162(3857):1024-6. doi: 10.1126/science.162.3857.1024.
Balb/c mouse embryo lines maintained in culture for over 200 generations under conditions that minimize cell-cell contact do not become tumorigenic. Lines cultivated under conditions where there is extensive cell contact become tumor-producing within 30 generations. The tissue-culture property that correlates best with tumorigenicity is the loss of contact inhibition of cell division.
在尽量减少细胞间接触的条件下培养超过200代的Balb/c小鼠胚胎细胞系不会变成致瘤性细胞系。在存在广泛细胞接触的条件下培养的细胞系在30代内会变成产生肿瘤的细胞系。与致瘤性相关性最好的组织培养特性是细胞分裂接触抑制的丧失。
