Watson B F, Dworkin M
J Bacteriol. 1968 Nov;96(5):1465-73. doi: 10.1128/jb.96.5.1465-1473.1968.
Crude extracts of both vegetative cells and glycerol-induced microcysts of Myxococcus xanthus contained the following enzyme activities: phosphofructokinase, phosphoglucoisomerase, fructose-1,6-diphosphatase, fructosediphosphate aldolase, glyceraldehyde-3-phosphate dehydrogenase, phosphopyruvate carboxylase, citrate synthase, isocitrate dehydrogenase, alpha-ketoglutarate dehydrogenase, succinate dehydrogenase, malate dehydrogenase, glucose-6-phosphate dehydrogenase, 6-phosphogluconate dehydrogenase, phosphoglucomutase, and uridine diphosphate glucose pyrophosphorylase. With the exception of isocitrate dehydrogenase, which was present at a fivefold higher concentration in microcysts, all activities in extracts from both types of cells were essentially equal. Hexokinase and pyruvate kinase could not be detected in extracts from either type of cell. Microcysts metabolized acetate at a lower rate than did vegetative cells. Most of this decrease was reflected in a substantial decrease in ability of microcysts to oxidize acetate to CO(2). In addition, microcysts and vegetative cells showed a different distribution of (14)C-label from incorporated acetate.
磷酸果糖激酶、磷酸葡萄糖异构酶、果糖-1,6-二磷酸酶、果糖二磷酸醛缩酶、甘油醛-3-磷酸脱氢酶、磷酸丙酮酸羧化酶、柠檬酸合酶、异柠檬酸脱氢酶、α-酮戊二酸脱氢酶、琥珀酸脱氢酶、苹果酸脱氢酶、葡萄糖-6-磷酸脱氢酶、6-磷酸葡萄糖酸脱氢酶、磷酸葡萄糖变位酶和尿苷二磷酸葡萄糖焦磷酸化酶。除了异柠檬酸脱氢酶在微囊肿中的浓度高五倍外，两种细胞提取物中的所有活性基本相等。在两种细胞类型的提取物中均未检测到己糖激酶和丙酮酸激酶。微囊肿代谢乙酸盐的速率低于营养细胞。这种下降大部分反映在微囊肿将乙酸盐氧化为CO₂的能力大幅下降。此外，微囊肿和营养细胞在掺入乙酸盐的¹⁴C标记分布上表现不同。
