O'Callaghan D J, Hyde J M, Gentry G A, Randall C C
J Virol. 1968 Aug;2(8):793-804. doi: 10.1128/JVI.2.8.793-804.1968.
Infection of exponential-phase suspension cultures of mouse fibroblast cells (L-M) with equine abortion virus (EAV) resulted in inhibition of cell growth and marked alterations in host metabolic processes. The synthesis of deoxyribonucleic acid (DNA) and ribonucleic acid was inhibited within 4 hr after infection and was suppressed by more than 90% by the time of maximal virus replication (14 to 18 hr). The overall rate of protein synthesis, however, was similar in uninfected and virus-producing cells as determined by measurements of net protein and isotope incorporation. The time course of viral DNA and protein synthesis and assembly into mature virus was determined with the inhibitors 5-fluorodeoxyuridine (FUdR) and cycloheximide, respectively. Thus, viral DNA synthesis was essentially completed at 14 hr, and viral protein and infectious virus synthesis was completed at 18 hr. Although the number of plaque-forming units (PFU) produced by FUdR-treated cells (10(3) to 10(4) PFU/ml) was at least 3 logs less than that produced by untreated cells, the yield of physical particles (as determined by electron microscopy) was approximately the same at 30 hr after infection. Besides being relatively non-infective, the particles produced in FUdR-treated cells appeared morphologically incomplete as they contained little or no nucleoid material.
用马流产病毒（EAV）感染处于指数生长期的小鼠成纤维细胞（L-M）悬浮培养物，导致细胞生长受到抑制，并使宿主代谢过程发生显著改变。感染后4小时内，脱氧核糖核酸（DNA）和核糖核酸的合成受到抑制，在病毒复制高峰期（14至18小时）时，其合成被抑制超过90%。然而，通过测量净蛋白和同位素掺入量确定，未感染细胞和产生病毒的细胞中蛋白质合成的总体速率相似。分别用抑制剂5-氟脱氧尿苷（FUdR）和环己酰亚胺确定了病毒DNA和蛋白质合成以及组装成成熟病毒的时间进程。因此，病毒DNA合成在14小时基本完成，病毒蛋白质和感染性病毒合成在18小时完成。尽管经FUdR处理的细胞产生的噬斑形成单位（PFU）数量（10³至10⁴ PFU/ml）比未处理细胞产生的至少少3个对数，但感染后30小时，物理颗粒的产量（通过电子显微镜确定）大致相同。除了相对无感染性外，在经FUdR处理的细胞中产生的颗粒在形态上似乎不完整，因为它们几乎不含或不含类核物质。
