Lawrence W C
J Virol. 1971 Jun;7(6):736-48. doi: 10.1128/JVI.7.6.736-748.1971.
Autoradiographic analyses of deoxyribonucleic acid (DNA) synthesis in randomly growing KB cell cultures infected with equine abortion virus (EAV) suggested that viral DNA synthesis was initiated only at times that coincided with the entry of noninfected control cells into the S phase of the cell cycle. Synchronized cultures of KB cells were infected at different stages of the cell cycle, and rates of synthesis of cellular and viral DNA were measured. When cells were infected at different times within the S phase, viral DNA synthesis was initiated 2 to 3 hr after infection. However, when cells in G1 and G2 were infected, the initiation of viral DNA synthesis was delayed and occurred only at times corresponding to the S phase. The times when viral DNA synthesis began were independent of the time of infection and differed by as much as 5 hr, depending on the stage of the cell cycle at which cells were infected. Viral one-step growth curves were also related to the S phase in a manner which indicated a relationship between the initiation of viral DNA synthesis and the S phase. These data support the concept that initiation of EAV DNA synthesis is dependent upon some cellular function(s) which is related to the S phase of the cell cycle.
对感染马流产病毒（EAV）的随机生长的KB细胞培养物中脱氧核糖核酸（DNA）合成的放射自显影分析表明，病毒DNA合成仅在与未感染的对照细胞进入细胞周期S期的时间一致时才开始。在细胞周期的不同阶段对KB细胞同步培养物进行感染，并测量细胞和病毒DNA的合成速率。当在S期内的不同时间感染细胞时，病毒DNA合成在感染后2至3小时开始。然而，当G1期和G2期的细胞被感染时，病毒DNA合成的起始被延迟，并且仅在对应于S期的时间发生。病毒DNA合成开始的时间与感染时间无关，并且相差多达5小时，这取决于细胞被感染时的细胞周期阶段。病毒一步生长曲线也以表明病毒DNA合成起始与S期之间关系的方式与S期相关。这些数据支持这样的概念，即EAV DNA合成的起始取决于与细胞周期S期相关的某些细胞功能。
