异戊烯基转移酶催化6,7-二氢香叶基焦磷酸合成10,11-二氢法尼基焦磷酸。
Synthesis of 10,11-dihydrofarnesyl pyrophosphate from 6,7-dihydrogeranyl pyrophosphate by prenyltransferase.
作者信息
Popják G, Holloway P W, Baron J M
出版信息
Biochem J. 1969 Feb;111(3):325-32. doi: 10.1042/bj1110325.
The syntheses of 6,7-dihydrogeraniol and of its pyrophosphate are described. It is shown that this analogue of geranyl pyrophosphate is a substrate for liver prenyltransferase and that the product synthesized by this enzyme from it and isopentenyl pyrophosphate is 10,11-dihydrofarnesyl pyrophosphate. The K(m) value for 6,7-dihydrogeranyl pyrophosphate was determined to be 1.11+/-0.19mum as compared with 4.34+/-1.71mum for geranyl pyrophosphate. The maximum reaction velocity with the artifical substrate was, however, only about one-fourth of that observed with geranyl pyrophosphate. The binding of isopentenyl pyrophosphate to the enzyme was not affected by the artificial substrate.
本文描述了6,7-二氢香叶醇及其焦磷酸酯的合成。结果表明，这种香叶基焦磷酸酯类似物是肝脏异戊烯基转移酶的底物，该酶由其与异戊烯基焦磷酸合成的产物是10,11-二氢法呢基焦磷酸。6,7-二氢香叶基焦磷酸的K(m)值测定为1.11±0.19μm，而香叶基焦磷酸的K(m)值为4.34±1.71μm。然而，与香叶基焦磷酸相比，人工底物的最大反应速度仅为其约四分之一。异戊烯基焦磷酸与该酶的结合不受人工底物的影响。
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