Olson E J, Cazort R J
J Gen Physiol. 1969 Mar;53(3):311-22. doi: 10.1085/jgp.53.3.311.
Both calcium and strontium could be transported actively from erythrocytes if adenosine triphosphate, guanosine triphosphate, or inosine triphosphate were included in the hypotonic medium used to infuse calcium or strontium into the cells. Acetyl phosphate and pyrophosphate were not energy sources for the transport of either ion. Neither calcium nor strontium transport was accompanied by magnesium exchange, and the addition of Mg(++) to the reaction medium in a final concentration of 3.0 mmoles/liter did not promote the transport of either ion. In the absence of nucleotide triphosphates, the addition of 1.5 mmoles/liter of Sr(++) to the reaction solution did not bring about active calcium transport and similarly 1.5 mmoles/liter of Ca(++) did not bring about active strontium transport. The inclusion of 1.5 mmoles/liter of Ca(++) or Sr(++) in the reaction medium did not interfere with the transport of the other ion when the erythrocytes were infused with adenosine triphosphate.
如果在用于将钙或锶注入细胞的低渗介质中加入三磷酸腺苷、三磷酸鸟苷或三磷酸肌苷，钙和锶都可以从红细胞中被主动转运。乙酰磷酸和焦磷酸都不是这两种离子转运的能量来源。钙和锶的转运都不伴随镁的交换，并且在反应介质中加入终浓度为3.0毫摩尔/升的Mg(++)也不会促进这两种离子的转运。在没有三磷酸核苷酸的情况下，向反应溶液中加入1.5毫摩尔/升的Sr(++)不会引起钙的主动转运，同样，1.5毫摩尔/升的Ca(++)也不会引起锶的主动转运。当红细胞用三磷酸腺苷灌注时，在反应介质中加入1.5毫摩尔/升的Ca(++)或Sr(++)不会干扰另一种离子的转运。
