猪、绵羊和鸡的小肠上皮细胞合成甘油三酯的过程。
Triglyceride synthesis by the small-intestinal epithelium of the pig, sheep and chicken.
作者信息
Bickerstaffe R, Annison E F
出版信息
Biochem J. 1969 Feb;111(4):419-29. doi: 10.1042/bj1110419.
Abstract
- A comparative study was made of triglyceride synthesis by the intestinal epithelium of pigs, sheep and chickens. In pig and chicken tissue both the glycerol 3-phosphate and the monoglyceride pathway of triglyceride synthesis were operative, but the former pathway predominated in sheep tissue. 2. The fatty acid specificity of the glycerol 3-phosphate pathway was studied in pig and sheep total-homogenate preparations. Maximum incorporation was obtained with myristic acid and palmitic acid under optimum conditions for each fatty acid. Lauric acid, myristic acid, oleic acid, linoleic acid and linolenic acid were inhibitory at concentrations above their optimum, but octanoic acid, decanoic acid, palmitic acid and stearic acid did not show this effect. 3. Subcellular fractionation located the glycerol 3-phosphate and monoglyceride pathways of triglyceride synthesis in the microsomes in all instances. Phosphatidate phosphohydrolase was associated with both the microsomes and the particle-free supernatant. 4. Glycerol 1-mono-oleate was incorporated into triglycerides to a greater extent than glycerol 1-mono-palmitate or glycerol 1-monostearate by microsomal preparations from pig and chicken. 5. A lipase specific for monoglycerides was detected in the particle-free supernatant of all the species examined.
摘要
- 对猪、羊和鸡的肠上皮细胞合成甘油三酯的过程进行了比较研究。在猪和鸡的组织中，甘油三酯合成的3-磷酸甘油途径和甘油单酯途径均起作用，但在羊的组织中前一种途径占主导地位。2. 在猪和羊的全匀浆制剂中研究了3-磷酸甘油途径的脂肪酸特异性。在每种脂肪酸的最佳条件下，肉豆蔻酸和棕榈酸的掺入量最高。月桂酸、肉豆蔻酸、油酸、亚油酸和亚麻酸在浓度高于其最佳浓度时具有抑制作用，但辛酸、癸酸、棕榈酸和硬脂酸未表现出这种效应。3. 亚细胞分级分离在所有情况下均将甘油三酯合成的3-磷酸甘油途径和甘油单酯途径定位在微粒体中。磷脂酸磷酸水解酶与微粒体和无颗粒上清液均有关联。4. 猪和鸡的微粒体制剂将甘油单油酸酯掺入甘油三酯的程度高于甘油单棕榈酸酯或甘油单硬脂酸酯。5. 在所有受试物种的无颗粒上清液中均检测到一种对甘油单酯具有特异性的脂肪酶。
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