Stokes G B, Poteat L W, Tove S B
Biochim Biophys Acta. 1975 Feb 20;380(2):245-56. doi: 10.1016/0005-2760(75)90010-7.
Microsome/cytosol preparations from adipose tissue of the mouse, pig, rat and chicken and from pig liver synthesize triacylglycerols containing a fatty acid distribution consistent with that in their respective fats. Since the incorporation of fatty acids depends on the presence of glycerol phospahte and no loss of tritium occurs during the incorporation of sn-[2-3 H]glycerol 3-phosphate into triacylglycerols, it would appear that the specific distribution is a property of the transacylases of the glycerophosphate pathway. The apparent Michaelis constant for sn-glycerol 3-phosphate, measured either by sn-glycerol 3-phosphate or palmitate incorporation, averaged 1.4-10-4 M both the mouse and pig adipose tissue enzyme systems.
从小鼠、猪、大鼠和鸡的脂肪组织以及猪肝制备的微粒体/胞质溶胶制剂能合成脂肪酸分布与各自脂肪中脂肪酸分布一致的三酰甘油。由于脂肪酸的掺入依赖于甘油磷酸的存在，并且在将sn-[2-³H]甘油3-磷酸掺入三酰甘油的过程中没有氚的损失，因此这种特定分布似乎是甘油磷酸途径转酰基酶的一种特性。通过sn-甘油3-磷酸或棕榈酸掺入法测定的sn-甘油3-磷酸的表观米氏常数，在小鼠和猪脂肪组织酶系统中平均为1.4×10⁻⁴M。
