Polheim D, David J S, Schultz F M, Wylie M B, Johnston J M
J Lipid Res. 1973 Jul;14(4):415-21.
The synthesis of phosphatidic acid and di- and triglycerides via the glycerol-3-phosphate pathway is markedly inhibited by 2-monooleyl ether in microsomal and whole cell preparations obtained from adipose and intestinal tissue. Monoglycerides are also inhibitors under conditions in which their hydrolysis is minimized. A correlation between inhibition by, and the hydrolysis of, monoglycerides has been demonstrated. 2-Monooleyl ether is the most effective inhibitor of the several mono- and di- ethers and esters studied. The specificity of the inhibition of glycerol-3-phosphate acylation by 2-monoethers or 2-monoesters has been demonstrated because microsomal NADH- and NADPH-cytochrome c reductase activities were not significantly inhibited. The reported control mechanism for triglyceride biosynthesis is discussed in relation to the regulation of fatty acid uptake and release in adipose tissue and the absorption and metabolism of triglycerides by the intestinal mucosa.
在从脂肪组织和肠道组织获得的微粒体和全细胞制剂中，2-单油酰醚可显著抑制通过甘油-3-磷酸途径合成磷脂酸、甘油二酯和甘油三酯。在单甘油酯水解最小化的条件下，单甘油酯也是抑制剂。已经证明了单甘油酯的抑制作用与其水解之间的相关性。在所研究的几种单醚、二醚和酯中，2-单油酰醚是最有效的抑制剂。由于微粒体NADH-和NADPH-细胞色素c还原酶活性未受到显著抑制，因此证明了2-单醚或2-单酯对甘油-3-磷酸酰化的抑制具有特异性。结合脂肪组织中脂肪酸摄取和释放的调节以及肠道黏膜对甘油三酯的吸收和代谢，讨论了所报道的甘油三酯生物合成的控制机制。
