Van Overbeek J, Loeffler J E, Mason M I
Science. 1967 Jun 16;156(3781):1497-9. doi: 10.1126/science.156.3781.1497.
Dormin (abscisin II), inhibits growth of Lemna minor cultures. At 1 part per million (3.8x10(-6)M), the culture appears nearly completely dormant but can be revived readily by transferring it to fresh medium free of dormin. The cytokinin benzyladenine, but not auxin or gibberellin, will counteract the dormin effect. Quantitative restoration of normal growth by cytokinin, however, can be achieved only if the dormin concentration does not exceed a critical level. Separation, after phenol-detergent extraction, of nucleic acids on methylated albumin kieselguhr columns showed suppression of nucleic acid synthesis by dormin in all fractions. Inhibition of the synthesis of the DNA fraction seems to precede that of RNA. Cytokinins reverse the process. They promote synthesis of all nucleic acid fractions, but again DNA seems to lead. Further work on the interaction of dormin with growth-promoting hormones might be facilitated by adopting the Monod model of allosteric transition, with, for example, DNA polymerase as the protein, dormin as the inhibitor, and cytokinin or other growth promoters as activators.
休眠素（脱落酸Ⅱ）抑制浮萍属微小浮萍培养物的生长。在百万分之一（3.8×10⁻⁶M）的浓度下，培养物几乎完全休眠，但将其转移到不含休眠素的新鲜培养基中即可轻易复苏。细胞分裂素苄基腺嘌呤能抵消休眠素的作用，而生长素或赤霉素则不能。然而，只有当休眠素浓度不超过临界水平时，细胞分裂素才能使生长恢复正常。用酚 - 去污剂提取后，在甲基化白蛋白硅藻土柱上分离核酸，结果表明休眠素对所有组分的核酸合成均有抑制作用。DNA组分合成的抑制似乎先于RNA。细胞分裂素可逆转这一过程。它们促进所有核酸组分的合成，但同样DNA似乎起主导作用。采用别构转变的莫诺德模型，例如以DNA聚合酶作为蛋白质，休眠素作为抑制剂，细胞分裂素或其他生长促进剂作为激活剂，可能有助于进一步研究休眠素与生长促进激素之间的相互作用。
