Vanderhoef L N, Stahl C A
Proc Natl Acad Sci U S A. 1975 May;72(5):1822-5. doi: 10.1073/pnas.72.5.1822.
A continuous growth apparatus was used to measure the effect of cytokinin on auxin-induced elongation. The soybean hypocotyl segments elicited a bi-phasic response to auxin that appeared to be two overlapping responses. The first response, which began 12 min after auxin addition, was not inhibited by cytokinin, even after long preincubation in cytokinin, but the second response to auxin, which began about 30 min after auxin addition, was completely inhibited by cytokinin. Such overlapping reactions are shown, depending on the amount of overlap, to yield a variety of summation reactions, many of which resemble rate-time curves that have been previously reported. We have shown that the transient first phase of auxin-induced elongation is very similar to acid-activated growth, while the second phase is long lasting and very likely identical to the long-term response to auxin, as extensively studied in Avena, soybean, and other elongating cells.
使用连续生长装置来测量细胞分裂素对生长素诱导伸长的影响。大豆下胚轴切段对生长素产生双相反应，这似乎是两种重叠的反应。第一种反应在添加生长素后12分钟开始，即使在细胞分裂素中长时间预孵育后也不受细胞分裂素的抑制，但对生长素的第二种反应在添加生长素后约30分钟开始，完全受细胞分裂素抑制。根据重叠量的不同，显示出这种重叠反应会产生多种总和反应，其中许多类似于先前报道的速率-时间曲线。我们已经表明，生长素诱导伸长的短暂第一阶段与酸激活生长非常相似，而第二阶段持续时间长，很可能与对生长素的长期反应相同，这在燕麦、大豆和其他伸长细胞中已有广泛研究。
