Block J H, Levine H L, Ayres J W
J Pharm Sci. 1979 May;68(5):605-8. doi: 10.1002/jps.2600680524.
The assay of suppositories containing pentobarbital and/or pyrilamine in a water-soluble polyethylene glycol base by high-pressure liquid chromatography is described. No extraction is required. The suppository is dissolved in the mobile phase. This solution is diluted with an internal standard stock solution containing phenobarbital. Chromatographic conditions include a C18 bonded microporous silica column and a mobile phase of 65% 4 x 10(-3) M n-butyl sodium sulfonate in 1% acetic acid and 35% acetonitrile. The procedure using commercial products gave results comparable to those obtained by GLC.
本文描述了采用高压液相色谱法测定以水溶性聚乙二醇为基质、含有戊巴比妥和/或吡拉明的栓剂。无需萃取。将栓剂溶解于流动相中。该溶液用含有苯巴比妥的内标储备溶液稀释。色谱条件包括使用C18键合微孔硅胶柱，流动相为65%的4×10⁻³M正丁基磺酸钠的1%乙酸溶液和35%的乙腈。使用市售产品的该方法所得结果与气相色谱法获得的结果相当。
