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[制备触珠蛋白的简易方法]

[Simple method for the preparation of haptoglobin].

作者信息

Schössler W, Zorn H, Patzelt D

出版信息

Acta Biol Med Ger. 1979;38(4):669-72.

PMID:43056
Abstract

The present paper deals with a method permitting the isolation of haptoglobin 2-2 from human serum or plasma. The haptoglobin is adsorbed to DEAE-cellulose at pH 5.1 by batching. The loaded cellulose is given into a column and the haptoglobin eluted by a 0.1 M to 0.15 M acetate buffer. The last step is a gel-chromatography on Sephadex G-200. Disc- and immunoelectrophoresis were used to test the purity. As by-product acid alpha1-glycoprotein can be obtained.

摘要

本文介绍了一种从人血清或血浆中分离触珠蛋白2-2的方法。通过分批处理,在pH 5.1条件下将触珠蛋白吸附到DEAE-纤维素上。将负载有触珠蛋白的纤维素装入柱中,并用0.1 M至0.15 M的醋酸盐缓冲液洗脱触珠蛋白。最后一步是在葡聚糖凝胶G-200上进行凝胶色谱分离。采用圆盘电泳和免疫电泳检测纯度。作为副产物,可以获得酸性α1-糖蛋白。

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