Shulman S, Milgrom F, Swanborg R H
Immunology. 1969 Jan;16(1):25-33.
The physical and chemical nature of the bovine brain BE antigen was studied, along with antigenic studies to verify the organ-specificity characteristics. These preparations were found to be quite heterogeneous physico-chemically, containing nucleic acids, carbohydrates, protein and possible lipid constituents. The extinction coefficients were found to be 12.0 (at 280 mμ) and 2.2 (at 540 mμ, biuret). Ultracentrifugal analysis revealed three major components, with extrapolated sedimentation coefficients of 8.60, 2.20 and 1.80. Electrophoretic analysis showed six (or more) components. Because of the heterogeneity chemical classification of the actual brain-specific antigen was determined by degradative studies, using chymotrypsin and periodate. Based on thermostability, a carbohydrate content of 4.6 per cent, and destruction by dilute chymotrypsin, the brain-specific antigenic component has been tentatively classified as a mucoprotein.
对牛脑BE抗原的物理化学性质进行了研究，并开展抗原研究以验证其器官特异性特征。发现这些制剂在物理化学性质上相当不均一，含有核酸、碳水化合物、蛋白质以及可能的脂质成分。消光系数分别为12.0（在280 mμ处）和2.2（在540 mμ处，双缩脲法）。超速离心分析显示有三个主要成分，外推沉降系数分别为8.60、2.20和1.80。电泳分析显示有六个（或更多）成分。由于其不均一性，通过使用胰凝乳蛋白酶和高碘酸盐进行降解研究来确定实际脑特异性抗原的化学分类。基于热稳定性、4.6%的碳水化合物含量以及被稀胰凝乳蛋白酶破坏的特性，脑特异性抗原成分暂被归类为一种粘蛋白。
