Georgopoulos C P, Bisig R, Magazin M, Eisen H, Court D
J Virol. 1979 Feb;29(2):782-8. doi: 10.1128/JVI.29.2.782-788.1979.
A novel phage lambda mutation, called dc10, which interferes with proper lambda head assembly has been isolated and characterized. Phage lambda carrying this mutation is (i) unable to form plaques at 30 or 37 degrees C but does so at 42 degrees C and (ii) unable to form plaques at 42 degrees C on pN-constitutive hosts. Both properties are due to dc10 since all phage revertants for one phenotype simultaneously lose the other phenotype and vice versa. The dc10 mutation has been mapped in the B gene and has been shown to be dominant over the corresponding wild-type product. At 30 degrees C the dc10 mutation results in the formation of abnormal petit lambda heads made up of pE, pB, pC, and pNu3. Under pN-constitutive conditions, the dc10 mutation results in the formation of abnormal petit lambda heads made of pE, X1, and X2 only. A model to explain the data is presented.
一种名为dc10的新型λ噬菌体突变体已被分离和鉴定，该突变体干扰了λ噬菌体头部的正常组装。携带这种突变的λ噬菌体具有以下特性：（i）在30或37摄氏度时无法形成噬菌斑，但在42摄氏度时可以形成；（ii）在pN组成型宿主上，42摄氏度时无法形成噬菌斑。这两种特性均归因于dc10，因为所有恢复一种表型的噬菌体回复体同时也失去了另一种表型，反之亦然。dc10突变已定位在B基因中，并已证明对相应的野生型产物具有显性作用。在30摄氏度时，dc10突变导致形成由pE、pB、pC和pNu3组成的异常小λ噬菌体头部。在pN组成型条件下，dc10突变导致仅由pE、X1和X2组成的异常小λ噬菌体头部的形成。本文提出了一个解释这些数据的模型。
