空的、头状颗粒在体外包装噬菌体λ DNA的活性。
Activity of empty, headlike particles for packaging of DNA of bacteriophage lambda in vitro.
作者信息
Hohn B, Hohn T
出版信息
Proc Natl Acad Sci U S A. 1974 Jun;71(6):2372-6. doi: 10.1073/pnas.71.6.2372.
Abstract
A precursor head of phage lambda is synthesized after induction of cells lysogenic for lambdaD(-)F(-) and lambdaA(-) (head-defective) mutants. This precursor head can be assayed by complementation in vitro and can be purified by CsCl gradient centrifugation and sucrose gradient sedimentation. The precursor head contains no DNA and has the same dimensions as the petit lambda particle. It can be packed with lambda DNA in an extract from induced Escherichia coli lysogenic for a lambdaE(-) mutant.
摘要
在诱导对λD(-)F(-)和λA(-)（头部缺陷型）突变体溶源的细胞后，会合成噬菌体λ的前体头部。这种前体头部可以通过体外互补进行检测，并可通过氯化铯梯度离心和蔗糖梯度沉降进行纯化。前体头部不含DNA，其尺寸与微小λ颗粒相同。在来自对λE(-)突变体溶源的诱导大肠杆菌提取物中，它可以用λDNA进行包装。
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