从粗糙脉孢菌中纯化一种催化组氨酸途径三步反应的三功能酶。
Purification of a trifunctional enzyme, catalysing three steps of the histidine pathway, from Neurospora crassa.
作者信息
Minson A C, Creaser E H
出版信息
Biochem J. 1969 Aug;114(1):49-56. doi: 10.1042/bj1140049.
Abstract
- A procedure is described for the purification of an enzyme from Neurospora crassa that has three catalytic functions. These are 1-N-(5'-phosphoribosyl)-ATP pyrophosphohydrolase, 1-N-(5'-phosphoribosyl)-AMP cyclohydrolase and histidinol dehydrogenase (l-histidinol-NAD oxidoreductase, EC 1.1.1.23), and are responsible for the catalysis of reactions 2, 3 and 10 in the histidine pathway. The ratio of these three catalytic activities remains approximately the same throughout the purification procedure. Evidence is presented that the purified preparations contain a single protein exhibiting association-dissociation equilibria.
摘要
- 本文描述了一种从粗糙脉孢菌中纯化一种具有三种催化功能的酶的方法。这三种催化功能分别是1-N-(5'-磷酸核糖基)-ATP焦磷酸水解酶、1-N-(5'-磷酸核糖基)-AMP环水解酶和组氨醇脱氢酶（L-组氨醇-NAD氧化还原酶，EC 1.1.1.23），它们负责催化组氨酸途径中的反应2、3和10。在整个纯化过程中，这三种催化活性的比例大致保持不变。有证据表明，纯化后的制剂含有一种表现出缔合-解离平衡的单一蛋白质。
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