大鼠肝线粒体中琥珀酸氧化对丙酮酸和 L-(+)-异柠檬酸氧化的抑制作用。
The inhibition of pyruvate and Ls(+)-isocitrate oxidation by succinate oxidation in rat liver mitochondria.
作者信息
König T, Nicholls D G, Garland P B
出版信息
Biochem J. 1969 Sep;114(3):589-96. doi: 10.1042/bj1140589.
Abstract
- The effects of succinate oxidation on pyruvate and also isocitrate oxidation by rat liver mitochondria were studied. 2. Succinate oxidation was without effect on pyruvate and isocitrate oxidation when respiration was maximally activated with ADP. 3. When respiration was partially inhibited by atractylate, succinate oxidation severely inhibited the oxidation of pyruvate and isocitrate. 4. This inhibitory effect of succinate was associated with a two- to three-fold increase in the reduction of mitochondrial NAD(+) but no change in the reduction of cytochrome b. 5. It is concluded that, in the partially energy-controlled state, respiration is more severely inhibited at the first phosphorylating site than at the other two. 6. The effects of succinate oxidation are compared with those of palmitoylcarnitine oxidation. It is concluded that a rapid flow of electrons directly into the respiratory chain at the level of cytochrome b is in itself inadequate to inhibit the oxidation of intramitochondrial NADH. 7. The effects of succinate oxidation on pyruvate oxidation were similar in rat heart and liver mitochondria.
摘要
- 研究了琥珀酸氧化对大鼠肝线粒体丙酮酸氧化以及异柠檬酸氧化的影响。2. 当用ADP最大程度激活呼吸作用时，琥珀酸氧化对丙酮酸和异柠檬酸氧化没有影响。3. 当呼吸作用被苍术苷部分抑制时，琥珀酸氧化严重抑制丙酮酸和异柠檬酸的氧化。4. 琥珀酸的这种抑制作用与线粒体NAD(+)还原增加两到三倍有关，但细胞色素b的还原没有变化。5. 得出结论，在部分能量控制状态下，呼吸作用在第一个磷酸化位点比在其他两个位点受到更严重的抑制。6. 将琥珀酸氧化的影响与棕榈酰肉碱氧化的影响进行了比较。得出结论，电子在细胞色素b水平直接快速流入呼吸链本身不足以抑制线粒体内NADH的氧化。7. 琥珀酸氧化对丙酮酸氧化的影响在大鼠心脏和肝脏线粒体中相似。
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