Jones A L, Schmucker D L, Mooney J S, Ockner R K, Adler R D
Lab Invest. 1979 Apr;40(4):512-7.
In an attempt to demonstrate the morphology of the bile secretory apparatus, male rats were restrained and maintained on an isocaloric diet with (experimental) and without (control) taurocholate, which was continuously infused via a duodenal cannula. This method of taurocholate administration promotes a 2-fold increase in the bile acid pool size and bile secretory rate and increases the transport maximum of taurocholate by approximately 50%. After 48 hours, the livers from both the control and experimental animals were perfusion-fixed and whole hepatocytes as well as pericanalicular cytoplasm (defined as a 1-micron. wide zone of cytoplasm adjacent to the bile canaliculus) in both centrolobular and periportal cells were subjected to a stereologic analysis. Although taurocholate infusion produced relatively few changes in the amounts of organelles or inclusionswithin hepatocytes, it caused highly significant increases in the amount ofGolgi-rich area, Golgi membranes, and the number of vesicles with diameters greater than 1000 A in the pericanalicular area of cytoplasm. In addition to these changes, which occurred in both central and periportal zones, decreases in the volume of lysosomes and the surface area of smooth surfaced endoplasmic reticulum were observed. These data provide new evidence that the "bile secretory apparatus" may encompass several hepatocellular components which include the Golgi complex and a vesicular transport system.
为了展示胆汁分泌装置的形态，将雄性大鼠固定，并给予等热量饮食，一组（实验组）添加牛磺胆酸盐，另一组（对照组）不添加，牛磺胆酸盐通过十二指肠插管持续输注。这种给予牛磺胆酸盐的方法可使胆汁酸池大小和胆汁分泌率增加两倍，并使牛磺胆酸盐的转运最大值增加约50%。48小时后，对对照组和实验组动物的肝脏进行灌注固定，对中央小叶和门周细胞中的整个肝细胞以及胆小管周围细胞质（定义为与胆小管相邻的1微米宽的细胞质区域）进行体视学分析。虽然输注牛磺胆酸盐对肝细胞内细胞器或内含物的数量产生的变化相对较少，但它导致胆小管周围细胞质区域富含高尔基体的区域、高尔基体膜的数量以及直径大于1000埃的囊泡数量显著增加。除了在中央和门周区域都出现的这些变化外，还观察到溶酶体体积和光滑内质网表面积减少。这些数据提供了新的证据，表明“胆汁分泌装置”可能包括几个肝细胞成分，其中包括高尔基体复合体和一个囊泡转运系统。
