正常大鼠和部分肝切除大鼠胆汁酸负荷后胆汁流量和胆汁脂质排泄的比较研究。
Comparative studies on bile flow and biliary lipid excretion after bile-acid loading in normal and partially hepatectomized rats.
作者信息
Hoshino M, Hirano A, Hayakawa T, Kamiya Y, Ohiwa T, Tanaka A, Kumai T, Katagiri K, Miyaji M, Takeuchi T
机构信息
First Department of Internal Medicine, Nagoya City University Medical School, Japan.
出版信息
Biochem J. 1995 Jan 15;305 ( Pt 2)(Pt 2):367-71. doi: 10.1042/bj3050367.
This study was performed to investigate sequential changes in bile secretion and biliary lipids after taurocholic acid (TCA) loading of regenerating rat liver. TCA was administered intravenously at stepwise-increasing doses to groups of non-operated control and partially hepatectomized rats, 24, 72 and 168 h after surgery. Bile flow, bile-acid output (BAO) and phospholipid output (PLO) (expressed per gram of liver) in partially hepatectomized rats increased more than in the controls. Using an isolated perfusion rat-liver system, TCA infusion was also carried out on groups of non-operated control and hepatectomized rats 72 h after operation. Again bile flow, BAO and PLO (expressed per gram of liver) were significantly higher in the partial hepatectomy case, mirroring the results obtained in vivo. When horseradish peroxidase (HRP) was pulse-loaded in isolated perfusion preparations, the second peak of biliary HRP secretion in hepatectomized rats was significantly higher than in controls. We conclude that increased bile-acid flow in partially hepatectomized rats is dependent upon acceleration of vesicular transport accompanying or following proliferation in regenerating livers.
本研究旨在探讨牛磺胆酸（TCA）负荷后再生大鼠肝脏胆汁分泌及胆汁脂质的序贯变化。分别在术后24、72和168小时，以逐步递增剂量静脉注射TCA于未手术对照组和部分肝切除大鼠组。部分肝切除大鼠的胆汁流量、胆汁酸输出量（BAO）和磷脂输出量（PLO）（以每克肝脏表示）的增加幅度大于对照组。使用离体灌注大鼠肝脏系统，在术后72小时也对未手术对照组和肝切除大鼠组进行TCA输注。同样，部分肝切除组的胆汁流量、BAO和PLO（以每克肝脏表示）显著更高，这与体内实验结果一致。当在离体灌注制剂中脉冲加载辣根过氧化物酶（HRP）时，肝切除大鼠胆汁HRP分泌的第二个峰值显著高于对照组。我们得出结论，部分肝切除大鼠胆汁酸流量增加取决于再生肝脏增殖伴随或之后囊泡转运的加速。
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