Zor U, Kaneko T, Schneider H P, McCann S M, Lowe I P, Bloom G, Borland B, Field J B
Proc Natl Acad Sci U S A. 1969 Jul;63(3):918-25. doi: 10.1073/pnas.63.3.918.
Hypothalamic extract, containing the releasing factors for anterior pituitary hormones, within minutes stimulated adenyl cyclase activity and adenosine 3':5'-cyclic phosphate (cyAMP) concentrations in rat anterior pituitary in vitro. Cerebral cortical extract was ineffective and hypothalamic extract had no effect on these parameters in posterior pituitary or thyroid. Prostaglandin E(1) also increased adenyl cyclase activity and cyAMP levels in anterior pituitary tissue. Although NaF augmented adenyl cyclase activity, it did not elevate cyAMP. Epinephrine, norepinephrine, histamine, serotonin, dopamine, and vasopressin did not increase either adenyl cyclase or cyAMP. The increased adenyl cyclase and cyAMP produced by hypothalamic extract was associated with greater luteinizing hormone release from anterior pituitary in vitro.
含有垂体前叶激素释放因子的下丘脑提取物,在数分钟内即可刺激大鼠垂体前叶的腺苷酸环化酶活性及3':5'-环磷酸腺苷(环磷酸腺苷)浓度。大脑皮质提取物无此作用,下丘脑提取物对垂体后叶或甲状腺的这些参数也无影响。前列腺素E(1)也可增加垂体前叶组织中的腺苷酸环化酶活性及环磷酸腺苷水平。虽然氟化钠可增强腺苷酸环化酶活性,但它不会提高环磷酸腺苷水平。肾上腺素、去甲肾上腺素、组胺、5-羟色胺、多巴胺和加压素均不会增加腺苷酸环化酶或环磷酸腺苷。下丘脑提取物所引起的腺苷酸环化酶及环磷酸腺苷增加,与体外垂体前叶释放更多促黄体生成素有关。