Sambrook J, Shatkin A J
J Virol. 1969 Nov;4(5):719-26. doi: 10.1128/JVI.4.5.719-726.1969.
The conversion of simian virus 40 (SV40) component II deoxyribonucleic acid to component I has been used to assay polynucleotide ligase in extracts of tissue culture cells. All cell types examined, including chicken, hamster, mouse, monkey, and human cells, contained adenosine triphosphate-dependent ligase. After infection of mouse embryo, monkey kidney, and HeLa cells with polyoma virus, SV40, and vaccinia virus, respectively, the enzyme activity increased, but its cofactor requirement was unchanged. In vaccinia virus-infected cells, the increased activity was localized in the cytoplasm. Ligase induction occurred in the presence of cytosine arabinoside but was prevented by puromycin. Rifampicin blocked the production of infectious vaccinia particles but had little effect on the induction of ligase.
利用猿猴病毒40(SV40)组分II脱氧核糖核酸向组分I的转化来测定组织培养细胞提取物中的多核苷酸连接酶。所检测的所有细胞类型,包括鸡、仓鼠、小鼠、猴和人细胞,都含有依赖三磷酸腺苷的连接酶。分别用多瘤病毒、SV40和痘苗病毒感染小鼠胚胎、猴肾和HeLa细胞后,酶活性增加,但其辅因子需求未变。在痘苗病毒感染的细胞中,增加的活性定位于细胞质中。连接酶的诱导在阿糖胞苷存在的情况下发生,但被嘌呤霉素阻止。利福平阻断传染性痘苗颗粒的产生,但对连接酶的诱导影响很小。