Girard M, Marty L, Manteuil S
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1267-71. doi: 10.1073/pnas.71.4.1267.
Brief labeling of cells infected with simian virus 40 with tritiated uridine resulted in the incorporation of part of the label into material that was soluble in 1 M NaCl and sensitive to KOH, with a buoyant density close to that of DNA. The properties of this material suggest that it represents single-stranded nascent molecules of messenger RNA of simian virus 40 hydrogen-bonded over a small portion of their length to viral DNA templates. The name "simian virus 40 transcriptional intermediate molecules" is suggested for these naturally occurring DNA.RNA hybrid molecules. The DNA in the hybrid seems to be in the form of replicative intermediate molecules.
用氚标记的尿苷对感染猿猴病毒40的细胞进行短暂标记，结果发现部分标记掺入到可溶于1M氯化钠且对氢氧化钾敏感、浮力密度接近DNA的物质中。这种物质的特性表明，它代表了猿猴病毒40信使核糖核酸的单链新生分子，这些分子在其长度的一小部分上与病毒DNA模板形成氢键。建议将这些天然存在的DNA.RNA杂交分子命名为“猿猴病毒40转录中间分子”。杂交体中的DNA似乎呈复制中间分子的形式。
