面包酵母中一种主要含肌醇脂质——甘露糖基二肌醇二磷酸神经酰胺的分离与部分特性分析
Isolation and partial characterization of a major inositol-containing lipid in baker's yeast, mannosyl-diinositol, diphosphoryl-ceramide.
作者信息
Steiner S, Smith S, Waechter C J, Lester R L
出版信息
Proc Natl Acad Sci U S A. 1969 Nov;64(3):1042-8. doi: 10.1073/pnas.64.3.1042.
Abstract
A polar lipid containing about 20 per cent of the lipid soluble inositol has been purified from cells of Saccharomyces cerevisiae. This compound labeled with (3)H-inositol, (32)P(i), or (14)C glucose was isolated from aqueous pyridine extracts by means of paper chromatography. Unlabeled compound was obtained by a differential solubility scheme. Analytical and degradation studies suggest that this compound can be formulated as a mannosyl-diinositol, diphosphoryl-ceramide. It can be converted by treatment with base or acid into a mannosylmonoinositol phosphoryl-ceramide, a composition which relates it to the basic structure of the phytoglycolipids.
摘要
一种含有约20%脂溶性肌醇的极性脂质已从酿酒酵母细胞中纯化出来。这种用³H-肌醇、³²P(i)或¹⁴C葡萄糖标记的化合物通过纸色谱法从吡啶水提取物中分离出来。未标记的化合物通过差异溶解度方案获得。分析和降解研究表明，这种化合物可以被拟定为甘露糖基-二肌醇、二磷酸神经酰胺。通过用碱或酸处理，它可以转化为甘露糖基单肌醇磷酸神经酰胺，这种组成使其与植物糖脂的基本结构相关。
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