Trevelyan W E
J Lipid Res. 1966 May;7(3):445-7.
Phosphatidyl inositol and a cardiolipin-like phospholipid survive the autolysis which is induced in yeast by treating it with toluene, whereas other phospholipids are extensively degraded. Lipid from autolyzed yeast was suspended in isopropanol to extract lecithin and neutral lipid. The insoluble phosphatidyl inositol and cardiolipin were then separated by chromatography on silicic acid.
磷脂酰肌醇和一种心磷脂样磷脂在酵母经甲苯处理诱导的自溶过程中得以留存,而其他磷脂则被大量降解。将自溶酵母中的脂质悬浮于异丙醇中以提取卵磷脂和中性脂质。然后通过在硅酸上进行色谱分离不溶性的磷脂酰肌醇和心磷脂。