对快速标记的大鼠肝脏核糖核酸的特性进行表征，该核糖核酸对硅藻土上甲基化白蛋白柱具有高亲和力。
Characterization of rapidly labelled rat liver ribonucleic acid showing high affinity for columns of methylated albumin on kieselguhr.
作者信息
Kunz W, Niessing J, Schnieders B, Sekeris C E
出版信息
Biochem J. 1970 Feb;116(4):563-7. doi: 10.1042/bj1160563.
Abstract
Most of the rapidly labelled RNA from rat liver submitted to column chromatography on methylated albumin on kieselguhr remains tightly bound to the column and can only be recovered by elution with m-ammonia. The tightly bound RNA is composed mainly of DNA-like RNA. The binding capacity is dependent not only on base composition but also on molecular size: the heavier RNA molecules show a greater affinity to the column than do the lower-molecular-weight components. Rapidly labelled mouse liver and Saccharomyces cerevisiae RNA show similar behaviour to rat liver RNA on columns of methylated albumin on kieselguhr.
摘要
将大鼠肝脏中快速标记的RNA在硅藻土上的甲基化白蛋白柱上进行柱色谱分析时，大部分RNA会紧密结合在柱上，只有用间氨水洗脱才能回收。紧密结合的RNA主要由类DNA RNA组成。结合能力不仅取决于碱基组成，还取决于分子大小：较重的RNA分子对柱的亲和力比较低分子量的组分更大。快速标记的小鼠肝脏和酿酒酵母RNA在硅藻土上的甲基化白蛋白柱上的行为与大鼠肝脏RNA相似。
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