锰离子对大鼠肝脏胞质溶胶提取物中谷氨酰胺合成酶活性的抑制作用。
Inhibition of glutamine synthetase activity by manganous ions in a cytosol extract of rat liver.
作者信息
Joseph S K, Bradford N M, McGivan J D
出版信息
Biochem J. 1979 Nov 15;184(2):477-80. doi: 10.1042/bj1840477.
Abstract
Glutamine synthetase activity in a cytosol extract of liver was inhibited non-competitively by Mn2+ ions. The apparent Ki for Mn2" in the presence of phosphate was 8 micro M. Inhibition of glutamine synthetase by intracellular Mn2+ may contribute to the very low rates of glutamine synthesis observed in perfused liver and isolated hepatocytes.
摘要
肝脏胞质溶胶提取物中的谷氨酰胺合成酶活性受到 Mn2+ 离子的非竞争性抑制。在存在磷酸盐的情况下，Mn2+ 的表观抑制常数（Ki）为 8 微摩尔。细胞内 Mn2+ 对谷氨酰胺合成酶的抑制作用可能是导致在灌注肝脏和分离的肝细胞中观察到谷氨酰胺合成速率极低的原因之一。
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