Martinsen J S
Can J Comp Med. 1970 Jan;34(1):13-9.
The effect of diethylaminoethyl (DEAE) dextran and agar overlay medium pH on a small-plaque (SP) and large-plaque (LP) foot-and-mouth disease virus (FMDV), type A, strain 119 (A119) was studied. The SP virus was inhibited under normal agar overlay but the addition of 100, 1,000 and 2,000 microg DEAE dextran/ml of agar overlay permitted plaque development. By using untreated and DEAE dextran-treated agar overlay medium, plaque formation by the SP virus was enhanced when the pH of agar medium was raised to a more alkaline level before overlay. Plaques formed by the LP virus were relatively uninhibited under the regular overlay but were larger in the presence of 1,000 microg DEAE dextran/ml. The enhancement of LP virus plaques occurred at various pH levels and was also inversely related to the hydrogen ion concentration of agar overlays; regular and DEAE dextrantreated alkaline overlays produced larger plaques.
研究了二乙氨基乙基（DEAE）葡聚糖和琼脂覆盖培养基的pH值对A型口蹄疫病毒（FMDV）119株（A119）小蚀斑（SP）和大蚀斑（LP）的影响。在正常琼脂覆盖条件下，SP病毒受到抑制，但在每毫升琼脂覆盖物中添加100、1000和2000微克DEAE葡聚糖可使蚀斑形成。通过使用未处理和经DEAE葡聚糖处理的琼脂覆盖培养基，当琼脂培养基的pH值在覆盖前提高到更碱性水平时，SP病毒的蚀斑形成得到增强。LP病毒形成的蚀斑在常规覆盖条件下相对不受抑制，但在每毫升含有1000微克DEAE葡聚糖时蚀斑更大。LP病毒蚀斑的增强在不同pH水平下均会发生，并且也与琼脂覆盖物的氢离子浓度呈负相关；常规和经DEAE葡聚糖处理的碱性覆盖物产生的蚀斑更大。
