Carp R I
J Virol. 1967 Oct;1(5):912-9. doi: 10.1128/JVI.1.5.912-919.1967.
Simian virus 40 (SV40) infection of human diploid cells failed to cause an enhanced production of thymidine kinase during the first 10 days after infection. Thymidine kinase activities from extracts of SV40-transformed cultures (human or simian) were considerably higher than the activity levels in extracts from the normal cells of origin. In addition, whereas the kinase activities obtained for human diploid cultures decreased as the cell sheet became confluent, the kinase activities for SV40-transformed human cells remained high after confluence was reached. Antisera obtained from hamsters bearing SV40 or adeno-7-SV40 hybrid virus tumors selectively inhibited enzyme from transformed sources (human or simian). Also, the antisera selectively inhibited enzyme extracted from SV40-lytically infected monkey cells. Sera from normal animals or from hamsters bearing polyoma tumors failed to inhibit enzymes from normal, SV40-transformed, or SV40-lytically infected cells. The Michaelis constant of partially purified enzyme from SV40-transformed cells was two to five times as high as that obtained for partially purified enzyme from human diploid cell cultures.
在感染后的头10天里，人二倍体细胞感染猿猴病毒40（SV40）并未导致胸苷激酶产量增加。SV40转化培养物（人或猿猴来源）提取物中的胸苷激酶活性显著高于原始正常细胞提取物中的活性水平。此外，人二倍体培养物的激酶活性随着细胞层汇合而降低，而SV40转化的人细胞在达到汇合后激酶活性仍保持较高水平。从携带SV40或腺病毒7 - SV40杂交病毒肿瘤的仓鼠获得的抗血清选择性地抑制来自转化来源（人或猿猴）的酶。同样，该抗血清也选择性地抑制从SV40裂解感染的猴细胞中提取的酶。正常动物或携带多瘤病毒肿瘤的仓鼠的血清未能抑制来自正常、SV40转化或SV40裂解感染细胞的酶。SV40转化细胞中部分纯化酶的米氏常数是从人二倍体细胞培养物中获得的部分纯化酶的两到五倍。
