Lewis A M, Rowe W P
J Virol. 1971 Feb;7(2):189-97. doi: 10.1128/JVI.7.2.189-197.1971.
The nondefective adenovirus 2 (Ad2)-simian virus 40 (SV40) hybrid virus, Ad2(+)ND(1), does not induce heat-labile SV40 T antigen but does induce a previously uncharacterized heat-stable SV40 antigen-the SV40 "U" antigen. This antigen is detectable by both immunofluorescence and complement fixation by using sera from hamsters with SV40 tumors. Sera from hamsters bearing SV40 tumors can be divided into two groups, those that react with both SV40 T and U antigens (T(+)U(+) sera) and those that react with SV40 T antigen only (T(+)U(-) sera). SV40 U-specific sera from monkeys immunized with Ad2(+)ND(1)-infected cells do not react with SV40 T antigen by immunofluorescence but do react with an antigen in the nucleus of SV40-transformed cells and with an early, cytosine arabinoside-resistant antigen present in the nucleus of SV40-infected cells. A heat-stable SV40 antigen detectable by complement fixation with T(+)U(+) hamster sera is present in extracts of SV40-induced hamster tumors and in cell packs of SV40-infected or -transformed cells. SV40 U-antigen synthesis by Ad2(+)ND(1) virus is partially sensitive to inhibitors of deoxyribonucleic acid synthesis, whereas U-antigen synthesis by SV40 virus is an early cytosine arabinoside-resistant event. As an early SV40 antigen differing from SV40 T antigen, U antigen may play a role in malignant transformation mediated by SV40.
无缺陷腺病毒2（Ad2）-猴病毒40（SV40）杂交病毒Ad2(+)ND(1)不诱导热不稳定的SV40 T抗原，但能诱导一种以前未被鉴定的热稳定SV40抗原——SV40“U”抗原。通过使用来自患有SV40肿瘤的仓鼠的血清，利用免疫荧光和补体结合试验均可检测到这种抗原。患有SV40肿瘤的仓鼠血清可分为两组，一组与SV40 T抗原和U抗原均发生反应（T(+)U(+)血清），另一组仅与SV40 T抗原发生反应（T(+)U(-)血清）。用Ad2(+)ND(1)感染的细胞免疫的猴子产生的SV40 U特异性血清，通过免疫荧光不与SV40 T抗原发生反应，但与SV40转化细胞的细胞核中的一种抗原以及SV40感染细胞的细胞核中存在的一种早期、对阿糖胞苷耐药的抗原发生反应。用T(+)U(+)仓鼠血清通过补体结合试验可检测到的热稳定SV40抗原，存在于SV40诱导的仓鼠肿瘤提取物以及SV40感染或转化细胞的细胞团中。Ad2(+)ND(1)病毒合成SV40 U抗原对脱氧核糖核酸合成抑制剂部分敏感，而SV40病毒合成U抗原是一个早期的阿糖胞苷耐药事件。作为一种不同于SV40 T抗原的早期SV40抗原，U抗原可能在SV40介导的恶性转化中发挥作用。
