Croce C M, Huebner K, Koprowski H
Proc Natl Acad Sci U S A. 1974 Oct;71(10):4116-9. doi: 10.1073/pnas.71.10.4116.
Somatic cell hybrids between mouse cells deficient in thymidine kinase [ATP:thymidine 5'-phosphotransferase (EC 2.7.1.75)] and two different monkey cell lines transformed by an adeno 7-SV40 hybrid have been produced using both a semiselective and a double selective procedure. Concordant segregation of the expression of SV40 T antigen with a specific monkey chromosome has been observed in all the mouse-monkey hybrid clones examined. Subcloning of three SV40 T antigen positive hybrid clones resulted in their segregation into SV40 T antigen-positive and negative subclones. Positive correlation between the SV40 T antigen and the same monkey chromosome has been observed in all the subclones examined.
利用半选择性和双重选择性程序，已产生了胸苷激酶[ATP：胸苷5'-磷酸转移酶(EC 2.7.1.75)]缺陷的小鼠细胞与由腺病毒7-SV40杂种转化的两种不同猴细胞系之间的体细胞杂种。在所有检测的小鼠-猴杂种克隆中，均观察到SV40 T抗原的表达与特定猴染色体的一致分离。对三个SV40 T抗原阳性杂种克隆进行亚克隆，结果它们分离为SV40 T抗原阳性和阴性亚克隆。在所有检测的亚克隆中，均观察到SV40 T抗原与同一猴染色体之间存在正相关。
