Romani R, Monadjem A
Proc Natl Acad Sci U S A. 1970 Jul;66(3):869-73. doi: 10.1073/pnas.66.3.869.
Isolated mitochondria were maintained metabolically active and coupled, i.e., capable of respiratory control, for several hours at 25 degrees C. An increase in respiratory control generally occurred during the first 4-8 hr of incubation followed thereafter by a decline. Longevity is a dynamic function as evidenced by its dependency on substrate and cofactors, thiamine pyrophosphate, in particular. Magnesium was also essential to longevity; coenzyme A, bovine serum albumin, and reducing agents were not. The findings are discussed in terms of cytoplasmic-mitochondrial interrelationships and mitochondrial autonomy.
分离出的线粒体在25摄氏度下可保持数小时的代谢活性和偶联状态，即具备呼吸控制能力。在孵育的最初4 - 8小时内，呼吸控制通常会增强，随后则会下降。寿命是一种动态功能，这一点从其对底物和辅因子（特别是硫胺素焦磷酸）的依赖性就可以看出。镁对寿命也至关重要；辅酶A、牛血清白蛋白和还原剂则并非如此。本文从细胞质 - 线粒体相互关系和线粒体自主性的角度对这些发现进行了讨论。
